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Animal-free Recombinant Human BMP-230 Publications

Catalog number: HZ-1128

HEK293 expressed Endotoxin-free Animal-component free

规格

单价

10ug

￥2000

100ug

￥15000

1000ug

￥55000

Format: Lyophilized

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Related Recombinant Proteins

Technical Specifications

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The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

Recombinant human BMP-2 (HZ-1128) stimulates dose-dependent induction of alkaline phosphatase production in the ATDC-5 mouse chondrogenic cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. ATDC-5 cells were treated with increasing concentrations of recombinant human BMP-2 for 72 hours before lysis and addition of pNPP. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 values range from 7.5-37.5 ng/ml.

GeneID:
650
Species:
Human
Expression:
HEK293
Activity:
7.5-37.5 ng/mL EC50
Purity:
>95%
Molecular Mass:
28 kDa, homodimer, glycosylated
Formulation
2x PBS + 6% Ethanol, See Certificate of Analysis for details
Species Reactivity:
human,mouse

Stability and Reconstitution

Stability and Storage:
Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20oC) and (-80oC). Upon reconstitution we recommend that the solution can be stored at (4oC) for short term or at (-20oC) to (-80oC) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.


Reconstitution:
Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 4 mM HCl containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Product Description

Product Description:

Animal-free Recombinant Human BMP-2 is expressed from human 293 cells as a disulfide linked homodimeric glycoprotein with an apparent molecular mass of 28 kDa. This cytokine is produced in a serum-free, chemically defined media. Production in human 293 cells offers authentic glycosylation, contributing to stability in cell growth media and other applications. BMPs (Bone Morphogenetic Proteins) belong to the TGF-beta superfamily of structurally related signaling proteins. BMP-2 is a potent osteoinductive cytokine, capable of inducing bone and cartilage formation in association with osteoconductive carriers such as collagen and synthetic hydroxyapatite.



Background:

Bone morphogenetic protein-2 (BMP-2) is a member of the transforming growth factor-beta (TGFB) superfamily. BMP-2 induces bone formation and regeneration during early embryonic development (PMID 16234975). It is involved in the hedgehog pathway, TGF beta signaling pathway, and cytokine-cytokine receptor interaction (PMID 26620161). Xeno-free Recombinant Human BMP-2 is expressed from human 293 cells as a disulfide linked homodimeric glycoprotein with an apparent molecular mass of 28 kDa.



Synonyms:
BMP 2, BMP 2A, BMP2, BMP-2, BMP2A, bone morphogenetic protein 2, Bone morphogenetic protein 2A



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