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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human BMP-2 protein

Activity

7.5-37.5 ng/mL EC50

Species Reactivity

human,mouse

Purity

>95%

Cat No : HZ-1128

Synonyms

BMP 2, BMP 2A, BMP2, BMP-2, BMP2A, bone morphogenetic protein 2, Bone morphogenetic protein 2A

Validation Data Gallery

  • The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

    The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

  • Recombinant human BMP-2 (HZ-1128) stimulates dose-dependent induction of alkaline phosphatase production in the ATDC-5 mouse chondrogenic cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. ATDC-5 cells were treated with increasing concentrations of recombinant human BMP-2 for 72 hours before lysis and addition of pNPP. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 values range from 7.5-37.5 ng/ml.

    Recombinant human BMP-2 (HZ-1128) stimulates dose-dependent induction of alkaline phosphatase production in the ATDC-5 mouse chondrogenic cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. ATDC-5 cells were treated with increasing concentrations of recombinant human BMP-2 for 72 hours before lysis and addition of pNPP. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 values range from 7.5-37.5 ng/ml.

Technical Specifications

GeneID 650
Species Human
Expression HEK293
Activity 7.5-37.5 ng/mL EC50
Purity >95%
Endotoxin<1 EU/µg
Accession NumberP21274
Molecular Mass 28 kDa, homodimer, glycosylated
Formulation 2x PBS + 6% Ethanol, See Certificate of Analysis for details
Species Reactivity human,mouse

Stability and Reconstitution

Stability and Storage Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 4 mM HCl containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Background

Bone morphogenetic protein-2 (BMP-2) is a member of the transforming growth factor-beta (TGFB) superfamily. BMP-2 induces bone formation and regeneration during early embryonic development (PMID 16234975). It is involved in the hedgehog pathway, TGF beta signaling pathway, and cytokine-cytokine receptor interaction (PMID 26620161). Xeno-free Recombinant Human BMP-2 is expressed from human 293 cells as a disulfide linked homodimeric glycoprotein with an apparent molecular mass of 28 kDa.

Synonyms

BMP 2, BMP 2A, BMP2, BMP-2, BMP2A, bone morphogenetic protein 2, Bone morphogenetic protein 2A

Publications

SpeciesTitle
rat

Carbohydr Polym

Aligned electrospun cellulose scaffolds coated with rhBMP-2 for both in vitro and in vivo bone tissue engineering.

Authors - Ximu Zhang
View Article
human

Tissue Eng Part C Methods

In Vitro Evaluation of Recombinant Bone Morphogenetic Protein-2 Bioactivity for Regenerative Medicine.

Authors - Stephanie L Fung
View Article

JCI Insight

Protein kinase G1 regulates bone regeneration and rescues diabetic fracture healing.

Authors - Nadine Schall
View Article
rat

Stem Cells Int

Transplantation Site Affects the Outcomes of Adipose-Derived Stem Cell-Based Therapy for Retinal Degeneration.

Authors - Chengyu Hu
View Article
human

ACS Biomater Sci Eng

Advanced Artificial Extracellular Matrices Using Amphiphilic Nanogel-Cross-Linked Thin Films To Anchor Adhesion Proteins and Cytokines.

Authors - Yoshihide Hashimoto
View Article
human

Biomed Res Int

A Novel Long Noncoding RNA, Lnc-OAD, Is Required for Bone Morphogenetic Protein 2- (BMP-2-) Induced Osteoblast Differentiation.

Authors - Zonggui Wang
View Article