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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human BMP-2 protein

Activity

7.5-37.5 ng/mL

Specific Activity

minimally 7.00 x 105 IU/mg

Species Reactivity

human,mouse,rat

Purity

>95%

Cat no : HZ-1128

Synonyms

BMP 2, BMP 2A, BMP2, BMP-2, BMP2A, bone morphogenetic protein 2, Bone morphogenetic protein 2A

Validation Data Gallery

  • Purity of recombinant human BMP-2 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human BMP-2 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • Recombinant human BMP-2 (HZ-1128) stimulates dose-dependent induction of alkaline phosphatase production in the ATDC-5 mouse chondrogenic cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. ATDC-5 cells were treated with increasing concentrations of recombinant human BMP-2 for 72 hours before lysis and addition of pNPP. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 values range from 7.5-37.5 ng/ml.

    Recombinant human BMP-2 (HZ-1128) stimulates dose-dependent induction of alkaline phosphatase production in the ATDC-5 mouse chondrogenic cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. ATDC-5 cells were treated with increasing concentrations of recombinant human BMP-2 for 72 hours before lysis and addition of pNPP. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 values range from 7.5-37.5 ng/ml.

Technical Specifications

GeneID 650
Species Human
Expression HEK293
Activity 7.5-37.5 ng/mL
Specific Activityminimally 7.00 x 105 IU/mg
Purity >95%
Endotoxin<1 EU/µg
Accession NumberP21274
Molecular Mass 17 kDa reduced, 28 kDa non-reduced, homodimer, glycosylated
Formulation 2x PBS + 6% Ethanol, See Certificate of Analysis for details
Species Reactivity human,mouse,rat

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 4 mM HCl containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Background

Bone morphogenetic protein-2 (BMP-2) is a member of the transforming growth factor-beta (TGFB) superfamily. BMP-2 induces bone formation and regeneration during early embryonic development (PMID 16234975). It is involved in the hedgehog pathway, TGF beta signaling pathway, and cytokine-cytokine receptor interaction (PMID 26620161). Xeno-free Recombinant Human BMP-2 is expressed from human 293 cells as a disulfide linked homodimeric glycoprotein with an apparent molecular mass of 28 kDa.

Synonyms

BMP 2, BMP 2A, BMP2, BMP-2, BMP2A, bone morphogenetic protein 2, Bone morphogenetic protein 2A

Publications

SpeciesTitle
rat

Adv Mater

Titanium Fiber Plates for Bone Tissue Repair.

Authors - Takashi Takizawa
View Article
human,mouse

Nat Commun

Deficiency of TMEM53 causes a previously unknown sclerosing bone disorder by dysregulation of BMP-SMAD signaling.

Authors - Long Guo
View Article
human

J Exp Med

Aberrant ZNF423 impedes B cell differentiation and is linked to adverse outcome of ETV6-RUNX1 negative B precursor acute lymphoblastic leukemia.

Authors - LenaHarder
View Article
human

Biomaterials

Dual growth factor loaded nonmulberry silk fibroin/carbon nanofiber composite 3D scaffolds for in vitro and in vivo bone regeneration.

Authors - DebokiNaskar
View Article
mouse

J Cell Biol

Pannexin 3 functions as an ER Ca(2+) channel, hemichannel, and gap junction to promote osteoblast differentiation.

Authors - MasakiIshikawa
View Article

Acta Biomater

Effect of calcium phosphate coating and rhBMP-2 on bone regeneration in rabbit calvaria using poly(propylene fumarate) scaffolds.

Authors - MahrokhDadsetan
View Article