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Histone H1.2 Polyclonal antibody
Histone H1.2 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
IF/ICC, IHC, IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | L02 cells, human testis tissue, Jurkat cells, MCF-7 cells, A375 cells, mouse thymus tissue |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells, HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
19649-1-AP targets Histone H1.2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | IF/ICC, IHC, IP, WB, ELISA |
Cited Applications | WB, IP, IF |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, Camelus bactrianus |
Immunogen | Histone H1.2 fusion protein Ag7503 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | histone cluster 1, H1c |
Synonyms | H1.2, H1F2, HIST1H1C, histone, histone cluster 1, H1c, Histone H1.2, Histone H1d |
Calculated Molecular Weight | 21 kDa |
Observed Molecular Weight | 32-33 kDa |
GenBank Accession Number | BC002649 |
Gene Symbol | HIST1H1C |
Gene ID (NCBI) | 3006 |
RRID | AB_10694432 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P16403 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for Histone H1.2 antibody 19649-1-AP | Download protocol |
IHC protocol for Histone H1.2 antibody 19649-1-AP | Download protocol |
IF protocol for Histone H1.2 antibody 19649-1-AP | Download protocol |
IP protocol for Histone H1.2 antibody 19649-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Oncogene Regulator of calcineurin 1 gene isoform 4 in pancreatic ductal adenocarcinoma regulates the progression of tumor cells. | ||
Oxid Med Cell Longev The Formation of Melanocyte Apoptotic Bodies in Vitiligo and the Relocation of Vitiligo Autoantigens under Oxidative Stress. | ||
J Neuroinflammation New insights into meningitic Escherichia coli infection of brain microvascular endothelial cells from quantitative proteomics analysis. | ||
Front Immunol HIST1H1C Regulates Interferon-β and Inhibits Influenza Virus Replication by Interacting with IRF3. |