验证数据展示
经过测试的应用
| Positive WB detected in | Calyculin A treated Jurkat cells, Jurkat cells |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Calyculin A treated Jurkat cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
30376-1-AP targets Phospho-RB1 (Ser807/811) in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | retinoblastoma 1 |
| 别名 | p RB1, p RB1 (Ser807/811), p RB1 Ser807/811, Phospho RB1, Phospho RB1 (Ser807/811) |
| 计算分子量 | 928 aa, 106 kDa |
| 观测分子量 | 110 kDa |
| GenBank蛋白编号 | BC039060 |
| 基因名称 | RB1 |
| Gene ID (NCBI) | 5925 |
| RRID | AB_3085351 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P06400 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RB1, also named as pp110, pRb and p105 Rb, belongs to the retinoblastoma protein (RB) family. Phosphorylation and dephosphorylation of RB1 is well known to be a key regulator in cell cycle progression in cancer cells. Phosphorylation of RB1 at multiple S/T residues, first by cyclin D-dependent kinases and subsequently by cyclin E-CDK2 in later G1 phase, completely inactivates RB1, enabling the release of E2F from RB1 complexes and activation of E2F-mediated transcription. Monomethylation at Lys810 by SMYD2 enhances phosphorylation at Ser807 and Ser811, and promotes cell cycle progression. (PMID: 22787429, PMID: 21115810, PMID: 32344731)
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for Phospho-RB1 (Ser807/811) antibody 30376-1-AP | Download protocol |
| IHC protocol for Phospho-RB1 (Ser807/811) antibody 30376-1-AP | Download protocol |
| WB protocol for Phospho-RB1 (Ser807/811) antibody 30376-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) The Compromised Fanconi Anemia Pathway in Prelamin A-Expressing Cells Contributes to Replication Stress-Induced Genomic Instability | ||
MedComm (2020) Phytosphingosine Alleviates Cigarette Smoke-Induced Bronchial Epithelial Cell Senescence in Chronic Obstructive Pulmonary Disease by Targeting the Free Fatty Acid Receptor 4. | ||
Adv Sci (Weinh) Biomimetic Nanomedicine for Senescence-Modulated Immune Activation Enhances Immunotherapy Efficacy in Hepatocellular Carcinoma. | ||
J Control Release Bioswitchable intracellular delivery of small activating RNA by tetrahedral framework nucleic acid: Application to p21-mediated anti-tumor therapy. | ||
Cell Death Discov Mitochondrial bioenergetics-SASP crosstalk determines senolytic efficacy in therapy-induced senescence. |
