NFKB1,p105,p50 Monoclonal antibody
NFKB1,p105,p50 Monoclonal Antibody for WB, IF/ICC, IHC, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human, mouse
Applications
WB, IF/ICC, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
2G1E3
验证数据展示
经过测试的应用
Positive WB detected in | LNCaP cells, HeLa cells, Jurkat cells, K-562 cells, THP-1 cells |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
Immunofluorescence (IF) | IF : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 3 publications below |
IF | See 3 publications below |
产品信息
66992-1-Ig targets NFKB1,p105,p50 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse samples.
Tested Applications | WB, IF/ICC, IHC, ELISA Application Description |
Cited Applications | WB, IF |
Tested Reactivity | Human, mouse |
Cited Reactivity | mouse |
Immunogen | NFKB1,p105,p50 fusion protein Ag5832 种属同源性预测 |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Full Name | nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 |
Synonyms | DKFZp686C01211, DNA binding factor KBF1, EBP 1, KBF1, NF kappa B, NFKB p105, NFKB p50, NFKB1, NFKB1,p105, NFKB1,p105,p50, p105, p50 |
Calculated Molecular Weight | 105 kDa |
Observed Molecular Weight | 50 kDa, 105 kDa |
GenBank Accession Number | BC051765 |
Gene Symbol | NFKB1 |
Gene ID (NCBI) | 4790 |
RRID | AB_2882309 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P19838 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for NFKB1,p105,p50 antibody 66992-1-Ig | Download protocol |
IHC protocol for NFKB1,p105,p50 antibody 66992-1-Ig | Download protocol |
IF protocol for NFKB1,p105,p50 antibody 66992-1-Ig | Download protocol |
FC protocol for NFKB1,p105,p50 antibody 66992-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
J Ethnopharmacol Sub-chronic exposure to realgar induces liver injury via upregulating the TXNIP/NLRP3 pathway and disturbing bile acid homeostasis in mice | ||
Chem Biol Drug Des Caffeic acid-grafted chitooligosaccharides downregulate MAPK and NF-kB in RAW264.7 cells | ||
Cell Commun Signal Semaphorin 7A interacts with nuclear factor NF-kappa-B p105 via integrin β1 and mediates inflammation | ||
Adv Sci (Weinh) NKRF in Cardiac Fibroblasts Protects against Cardiac Remodeling Post-Myocardial Infarction via Human Antigen R |