验证数据展示
经过测试的应用
| Positive WB detected in | Transfected HEK-293 cells |
| Positive IF/ICC detected in | Transfected HEK-293 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
68088-1-Ig targets mCherry in WB, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with recombinant protein samples.
| Tested Applications | WB, IF/ICC, ELISA Application Description |
| Cited Applications | WB, IF, IP, CoIP |
| Tested Reactivity | recombinant protein |
| Cited Reactivity | human, pig |
| Immunogen | mCherry fusion protein Ag25320 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | mCherry |
| Synonyms | mCherry |
| Calculated Molecular Weight | 27 kDa |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_2918825 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
mCherry is a fluorophore (a fluorescent protein) used in biotechnology as a tracer to follow the flow of fluids, as a marker when tagged to molecules and cell components. mCherry is the second generation monomeric red fluorescent protein that have improved brightness and photostability. mCherry and the majority of red fluorescent proteins derive from a protein isolated from Discosoma sp. mCherry is a monomeric fluorescent construct with peak absorption/emission at 587 nm and 610 nm, respectively.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for mCherry antibody 68088-1-Ig | Download protocol |
| IF protocol for mCherry antibody 68088-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Open Biol Coiled-coil-mediated dimerization of Atg16 is required for binding to the PROPPIN Atg21 | ||
J Virol Swine RNF5 positively regulates the antiviral activity of IFITM1 by mediating the degradation of ABHD16A | ||
J Biol Chem Porcine IKKε is involved in the STING-induced type I IFN antiviral response of the cytosolic DNA signaling pathway |