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NeutraControl IL-27 Monoclonal antibody

NeutraControl IL-27 Monoclonal Antibody for ELISA, Non-Neutralization

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

ELISA, Non-Neutralization

Conjugate

Unconjugated

CloneNo.

2F8E2

Cat no : 69534-1-Ig

Print datasheet

Synonyms

IL 27, IL 27 A, IL 27 subunit alpha, IL 27A, IL27, IL27 A, IL27A, IL27p28, IL30, interleukin 27, Interleukin 27 subunit alpha, p28

验证数据展示

  • Non-Neutralization experiment of OCI-AML5 cells using 69534-1-Ig

    Non-Neutralization experiment of OCI-AML5 cells using 69534-1-Ig

    Recombinant human IL-27(Cat.NO. HZ-1275) stimulates proliferation of OCI-AML5 cells in a dose-dependent manner (blue curve, refer to bottom X-left Y). The activity of human IL-27 (250 ng/mL) is neutralized by mouse anti-human IL-27 monoclonal antibody 69034-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 0.4-1 μg/mL.The control mouse anti-human IL-27 monoclonal antibody 69534-1-Ig could recognize human IL-27, but could not neutralize human IL-27 at serial does at the range of 0.0033-9μg/mL(green curve, refer to top X-right Y).

  • ELISA experiment of Recombinant protein using 69534-1-Ig

    ELISA experiment of Recombinant protein using 69534-1-Ig

    Indirect ELISA was carried out by coating recombinant Human IL-27 (Cat.NO. HZ-1275) at 70 ng/well followed by blocking and adding serial diluted IL-27 antibody 69034-1-Ig and 69534-1-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm.

产品信息

69534-1-Ig targets IL-27 in ELISA, Non-Neutralization applications and shows reactivity with Human samples.

Tested Applications ELISA, Non-Neutralization Application Description
Tested Reactivity Human
Immunogen human Humankine IL-27 protein HZ-1275 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name interleukin 27
Synonyms IL 27, IL 27 A, IL 27 subunit alpha, IL 27A, IL27, IL27 A, IL27A, IL27p28, IL30, interleukin 27, Interleukin 27 subunit alpha, p28
Gene Symbol IL-27
Gene ID (NCBI) 246778
Conjugate Unconjugated
Form Lyophilized Powder
Purification MethodProtein A purification
UNIPROT IDQ8NEV9
Storage Buffer Sterile PBS.
Endotoxin<0.1 EU/μg
ReconstitutionThis product was lyophilized from a 0.2 μm filtered solution in PBS. Reconstitute at 1.0 mg/mL in sterile H2O before use.
Stability and StorageLyophilized antibodies are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.

背景介绍

IL-27 is a heterodimeric cytokine composed of two subunits: IL-27a and IL-27b. IL-27 acts on various cell types, including T cells, B cells, macrophages, dendritic cells, natural killer (NK) cells and non-hematopoietic cells. IL-27 plays a critical role in the early regulation of T helper type 1 initiation, and enhances proliferation of naive CD4+T cells and naive B cells. It, however, also exerts anti-inflammatory functions by inhibiting the development of Th17 cells and inducing IL-10 producing type 1 regulatory T cells. IL-27 is a potentially promising cytokine for therapeutic approaches on various human diseases.

This antibody is a neutralizing control antibody for IL-27, the immunogen is the same as Neutrakine 69034-1-Ig but could not neutralize human IL-27.