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验证数据展示

  • WB analysis using 81538-1-RR

    WB analysis using 81538-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81538-1-RR (VDAC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 81538-1-RR

    IHC staining of mouse liver using 81538-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 81538-1-RR (VDAC1/Porin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with VDAC1 antibody included
  • FC experiment of HepG2 using 81538-1-RR

    FC experiment of HepG2 using 81538-1-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug VDAC1/Porin Recombinant antibody (81538-1-RR, Clone:2F4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of human liver using 81538-1-RR

    IF Staining of human liver using 81538-1-RR

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using VDAC1/Porin antibody (81538-1-RR, Clone: 2F4 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

VDAC1/Porin Recombinant antibody

VDAC1/Porin Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, FC (Intra), ELISA
Cat No. 81538-1-RR

产品说明书

CloneNo. 2F4
引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, FC (Intra), ELISA

别名

Porin, VDAC1, Porin 31HL, PORIN 31 HL, Plasmalemmal porin

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMDA-MB-231 cells, HeLa cells, LNCaP cells, Jurkat cells, K-562 cells, NIH/3T3 cells, HSC-T6 cells
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman liver tissue
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy VDAC1 Ready-To-Use IHC Kit. VDAC1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

81538-1-RR targets VDAC1/Porin in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, FC (Intra), ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 voltage-dependent anion channel 1
别名 Porin, VDAC1, Porin 31HL, PORIN 31 HL, Plasmalemmal porin
计算分子量 31 kDa
观测分子量 31 kDa
GenBank蛋白编号NM_003374
基因名称 Porin
Gene ID (NCBI) 7416
RRIDAB_2935560
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP21796
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VDAC1, also named as VDAC, porin 31HM, porin 31HL and plasmalemmal porin, belongs to the eukaryotic mitochondrial porin family. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV, to form a channel through the mitochondrial outer membrane and also the plasma membrane. Unlike other membrane transport proteins, porins are large enough to allow passive diffusion. Studies have shown that VDAC1 is subject to both phosphorylation and acetylation (PMID: 23233904). The apparent molecular weight of VDAC1 is 30-37 kDa (PMID: 14573604; 23754752; 25681439). Hypoxic conditions were found to trigger cleavage of the VDAC1 C-terminal to yield a 26-kDa truncated but active form (PMID: 22389449; 23233904). This antibody is specific to VDAC1.

实验方案

Product Specific Protocols
WB protocol for VDAC1/Porin antibody 81538-1-RRDownload protocol
IHC protocol for VDAC1/Porin antibody 81538-1-RRDownload protocol
IF protocol for VDAC1/Porin antibody 81538-1-RRDownload protocol
FC protocol for VDAC1/Porin antibody 81538-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

J Nutr Biochem

Iron-frataxin involved in the protective effect of quercetin against alcohol-induced liver mitochondrial dysfunction

Authors - Jingjing Liu
View Article
ratWB

Int J Nanomedicine

Mitochondria-Rich Microvesicles Alleviate CNI ED by Transferring Mitochondria and Suppressing Local Ferroptosis

Authors - Zhenkang Liang
View Article