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验证数据展示

  • WB analysis using 83288-2-RR

    WB analysis using 83288-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83288-2-RR (NUP85 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of MCF-7 using 83288-2-RR

    FC experiment of MCF-7 using 83288-2-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug NUP85 Recombinant antibody (83288-2-RR, Clone:240116E10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83288-2-RR

    Affinity and Kinetic Characterization of 83288-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83288-2-RR against Human NUP85 were performed. The affinity constant is 27.3 nM.

NUP85 Recombinant antibody

NUP85 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83288-2-RR

产品说明书

CloneNo. 240116E10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

Nucleoporin Nup85, Nucleoporin Nup75, nucleoporin 85kDa, FROUNT, 85 kDa nucleoporin

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, HeLa cells, K-562 cells, HepG2 cells, COLO 320 cells
Positive FC (Intra) detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy NUP85 Ready-To-Use IHC Kit. NUP85 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83288-2-RR targets NUP85 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 NUP85 fusion protein Ag7086 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 nucleoporin 85kDa
别名 Nucleoporin Nup85, Nucleoporin Nup75, nucleoporin 85kDa, FROUNT, 85 kDa nucleoporin
计算分子量 75 kDa
观测分子量70 kDa
GenBank蛋白编号BC000697
基因名称 NUP85
Gene ID (NCBI) 79902
RRIDAB_3670955
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9BW27
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nucleoporin 85 (Nup85) (also known as FROUNT) is a protein that in humans is encoded by the NUP85 gene. This gene encodes a protein component of the Nup107-160 subunit of the nuclear pore complex. Nuclear pore complexes are embedded in the nuclear envelope and promote bidirectional transport of macromolecules between the cytoplasm and nucleus. The encoded protein can also bind to the C-terminus of chemokine (C-C motif) receptor 2 (CCR2) and promote chemotaxis of monocytes, thereby participating in the inflammatory response.

实验方案

Product Specific Protocols
WB protocol for NUP85 antibody 83288-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols