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SARS-CoV-2 Membrane Glycoprotein Polyclonal antibody

SARS-CoV-2 Membrane Glycoprotein Polyclonal Antibody for ELISA

Host / Isotype

Rabbit / IgG

Reactivity

virus

Applications

ELISA

Conjugate

Unconjugated

Cat no : 28882-1-AP

Print datasheet

Synonyms

2019-nCOV membrane glycoprotein, M protein, membrane glycoprotein

验证数据展示

  • ELISA experiment of Recombinant protein using 28882-1-AP

    ELISA experiment of Recombinant protein using 28882-1-AP

    COVID-19 M Protein Antibody (28882-1-AP) tested by ELISA. COVID-19 M Protein(101-222aa ) were coated onto microtiter plates at 0.15 µg/well and then incubated with a dilution series of COVID-19 M Protein Antibody (28882-1-AP). Bound antibodies were detected with HRP conjugated Goat anti-Rabbit IgG followed by incubation with HRP Substrate and teminated with H2SO4, then measuring the resulting absorbance at 450 nm.

经过测试的应用

Positive ELISA detected inRecombinant protein

推荐稀释比

ApplicationDilution
Enzyme-linked Immunosorbent Assay (ELISA)ELISA : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28882-1-AP targets SARS-CoV-2 Membrane Glycoprotein in ELISA applications and shows reactivity with virus samples.

Tested Applications ELISA Application Description
Tested Reactivity virus
Immunogen SARS-CoV-2 Membrane Glycoprotein fusion protein Ag30691 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name COVID-19 M Protein
Synonyms 2019-nCOV membrane glycoprotein, M protein, membrane glycoprotein
GenBank Accession NumberNC_045512
Gene Symbol SARS-CoV-2 Membrane Protein
Gene ID (NCBI) 43740571
RRIDAB_2881226
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP0DTC5
Storage Buffer PBS with 0.02% sodium azide, 50% glycerol pH 7.3 and 0.05% BSA
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

In the coronaviruses, the membrane (M) protein was reported to be the most abundant viral protein expressed during infection and a key protein in the assembly of both naked and enveloped virus particles (PMID: 11967315). The M glycoprotein is conserved across the β-coronaviruses. The multiple sequence alignment shows a remarkable similarity (98% identity) among the Sars-CoV-2 M variants and the sequences from Bat and Pangolin isolates (PMID:32596311). The M protein is predicted to contain a triple-spanning transmembrane (TM) region, a single N-glycosylation site near its N-terminus that is in the exterior of the virion, and a long C-terminal region in the interior (PMID: 15626342).

发表文章

SpeciesApplicationTitle

J Virol

Palmitoylation of SARS-CoV-2 Envelope protein is central to virus particle formation

Authors - Zhaohuan Wang
View Article