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验证数据展示

  • WB analysis of K562 using 60246-1-Ig (same clone as 60246-1-PBS)

    WB analysis of K562 using 60246-1-Ig (same clone as 60246-1-PBS)

    WB result of 4EBP1 antibody (60246-1-Ig, 1:4000) with si-Control and si-4EBP1 transfected K562 cells. This data was developed using the same antibody clone with 60246-1-PBS in a different storage buffer formulation.

  • WB analysis of K-562 using 60246-1-Ig (same clone as 60246-1-PBS)

    WB analysis of K-562 using 60246-1-Ig (same clone as 60246-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 60246-1-Ig (4EBP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60246-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 60246-1-Ig (same clone as 60246-1-PBS)

    IF Staining of HeLa using 60246-1-Ig (same clone as 60246-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed HeLa cells using 60246-1-Ig(4EBP1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60246-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 60246-1-Ig (same clone as 60246-1-PBS)

    IHC staining of human pancreas cancer using 60246-1-Ig (same clone as 60246-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60246-1-Ig (4EBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60246-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EIF4EBP1 antibody included
  • IHC staining of human colon cancer using 60246-1-Ig (same clone as 60246-1-PBS)

    IHC staining of human colon cancer using 60246-1-Ig (same clone as 60246-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60246-1-Ig (4EBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60246-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EIF4EBP1 antibody included
  • IHC staining of human colon cancer using 60246-1-Ig (same clone as 60246-1-PBS)

    IHC staining of human colon cancer using 60246-1-Ig (same clone as 60246-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60246-1-Ig (4EBP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60246-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EIF4EBP1 antibody included
  • Featured Product
  • KD/KO验证

4EBP1 Monoclonal antibody, PBS Only

4EBP1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 60246-1-PBS

产品说明书

CloneNo. 2C3F3

宿主/亚型

Mouse / IgG2b

种属反应性

human, pig

应用

WB, IHC, IF/ICC, ELISA

别名

EIF4EBP1, eIF4E-binding protein 1, eIF4E binding protein 1, BP 1, 4E-BP1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy EIF4EBP1 Ready-To-Use IHC Kit. EIF4EBP1 primary antibody included.

产品信息

60246-1-PBS targets 4EBP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, pig samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, pig
免疫原 4EBP1 fusion protein Ag18985 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 eukaryotic translation initiation factor 4E binding protein 1
别名 EIF4EBP1, eIF4E-binding protein 1, eIF4E binding protein 1, BP 1, 4E-BP1
计算分子量 118 aa, 12 kDa
观测分子量 15-24 kDa
GenBank蛋白编号BC004459
基因名称 EIF4EBP1
Gene ID (NCBI) 1978
RRIDAB_2881368
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ13541
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Eukaryotic translation initiation factor 4E-binding protein 1(4EBP1), is a member of 4EBPs family, which regulate the translation of a subset of mRNA by competing with eIF4G for binding to eIF4E, thus preventing the assembly of the eIF4F complex. The eIF4F facilitates the recruitment of other translation initiation factors to form the complex and then initiates cap-dependent translation.4EBP1 also mediates the regulation of protein translation by growth factors, hormones and other stimuli that signal through the MAP kinase and mTORC1 pathways. There are three forms of 4EBP1, alpha, beta and gamma.typical pattern seen on Western blots for phosphorylated heat-acid stabled protein 4EBP1 from rat gastrocnemius muscle. Three distinct bands are noted, with the most rapidly migrating band having an apparent molecular mass of ; 20 kDa and the slowest migrating band of; 24 kDa. The 3 bands are designated by convention asalpha, beta and gamma. (PMID: 10913029)