验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293 cells, NCI-H1299 cells |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse skeletal muscle tissue |
| Positive IF/ICC detected in | HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15129-1-AP targets ABLIM1 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IF-P, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | ABLIM1 fusion protein Ag7258 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | actin binding LIM protein 1 |
| 别名 | ABLIM, abLIM-1, actin binding LIM protein 1, Actin-binding double zinc finger protein, Actin-binding LIM protein 1 |
| 计算分子量 | 88 kDa |
| 观测分子量 | 48-52 kDa, 105-110 kDa |
| GenBank蛋白编号 | BC002448 |
| 基因名称 | ABLIM1 |
| Gene ID (NCBI) | 3983 |
| RRID | AB_2257767 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O14639 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ABLIM1 (actin-binding LIM protein 1) is a member of the LIM-domain protein family, mediating interactions between actin filaments and cytoplasmic targets. ABLIM1 has been reported as a cell cortex protein specifically in non-erythrocytes, and is critical for the formation of dense interwoven cortical actin meshwork. Due to the alternative splicing, ABLIM1 has multiple isoforms with predicted masses of 52, 70, and 88 kDa, respectively(PMID: 9245787). The phosphorylation or binding with SUMO2 may result in higher MW.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ABLIM1 antibody 15129-1-AP | Download protocol |
| IHC protocol for ABLIM1 antibody 15129-1-AP | Download protocol |
| IF protocol for ABLIM1 antibody 15129-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Cell Biol miR-129-3p controls cilia assembly by regulating CP110 and actin dynamics.
| ||
J Cell Physiol Actin binding LIM 1 (abLIM1) negatively controls osteoclastogenesis by regulating cell migration and fusion.
| ||
Int J Biol Sci Rictor promotes cell migration and actin polymerization through regulating ABLIM1 phosphorylation in Hepatocellular Carcinoma.
| ||
Mol Biol Cell Spatial proximity of proteins surrounding zyxin under force-bearing conditions. | ||
medRxiv Antigenic responses are hallmarks of fibrotic interstitial lung diseases independent of underlying etiologies | ||
