• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 28868-1-AP

    WB analysis using 28868-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28868-1-AP (ACE2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 28868-1-AP

    IHC staining of human kidney using 28868-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28868-1-AP (ACE2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ACE2 antibody included
  • IHC staining of human kidney using 28868-1-AP

    IHC staining of human kidney using 28868-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28868-1-AP (ACE2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ACE2 antibody included
  • WB analysis of HeLa using 28868-1-AP

    WB analysis of HeLa using 28868-1-AP

    WB result of ACE2 antibody (28868-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACE2 transfected HeLa cells.

  • IF Staining of mouse kidney using 28868-1-AP

    IF Staining of mouse kidney using 28868-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using ACE2 antibody (28868-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse kidney using 28868-1-AP

    IF Staining of mouse kidney using 28868-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using ACE2 antibody (28868-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

ACE2 Polyclonal antibody

ACE2 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 28868-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, ELISA

别名

Angiotensin-converting enzyme homolog, Angiotensin-converting enzyme 2, Angiotensin converting enzyme 2, ACE-related carboxypeptidase, ACEH

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inmouse kidney tissue, HeLa cells, mouse testis tissue, rat testis tissue
Positive IHC detected inhuman kidney tissue, human colon cancer tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse kidney tissue
Planning an IHC experiment? We recommend our IHCeasy ACE2 Ready-To-Use IHC Kit. ACE2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28868-1-AP targets ACE2 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ACE2 fusion protein Ag30683 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
别名 Angiotensin-converting enzyme homolog, Angiotensin-converting enzyme 2, Angiotensin converting enzyme 2, ACE-related carboxypeptidase, ACEH
计算分子量 805 aa, 92 kDa
观测分子量92, 120 kDa
GenBank蛋白编号BC048094
基因名称 ACE2
Gene ID (NCBI) 59272
RRIDAB_2881224
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BYF1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACE2 (Angiotensin-converting enzyme 2), also named as ACEH, is a zinc metalloprotease of the ACE family and a critical regulator of the reninangiotensin system. ACE2 has a more restricted tissue distribution than ACE, being found predominantly in the heart, kidneys, and testes although low levels have been detected in a variety of tissues (PMID:15983030). ACE2 has been shown to be a functional receptor of the human coronaviruses SARS-CoV and SARS-CoV-2 (PMID: 32142651). The expression level and expression pattern of human ACE2 in different tissues might be critical for the susceptibility, symptoms, and outcome of 2019-nCoV/SARS-CoV-2 infection (PMID: 32133153). It can be used as a potential therapeutic target of SARS-CoV-2 (PMID: 32125455). The calculated molecular weight of ACE2 is 92kDa but it migrates to 120kDa due to N-glycosylation (PMID:16166094). Sometimes, several cleaved fragments can also be detected as 75kDa, 50 kDa or 37kDa (PMID: 29561187, 22009550, 30759273). It has 2 isoforms produced by alternative splicing. This antibody is specific to ACE2. The location of ACE2 is membrane and cytoplasm, however it accumulates in the nucleus during the mitosis (PMID: 1730413/PMID: 18292088). 28868-1-AP is specific for ACE2, and its antigen region is 30-356aa.

实验方案

Product Specific Protocols
WB protocol for ACE2 antibody 28868-1-APDownload protocol
IHC protocol for ACE2 antibody 28868-1-APDownload protocol
IF protocol for ACE2 antibody 28868-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols