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验证数据展示

  • WB analysis using 28868-1-AP

    WB analysis using 28868-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28868-1-AP (ACE2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 28868-1-AP

    IHC staining of human kidney using 28868-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28868-1-AP (ACE2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ACE2 antibody included
  • IHC staining of human kidney using 28868-1-AP

    IHC staining of human kidney using 28868-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28868-1-AP (ACE2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ACE2 antibody included
  • WB analysis of HeLa using 28868-1-AP

    WB analysis of HeLa using 28868-1-AP

    WB result of ACE2 antibody (28868-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACE2 transfected HeLa cells.

  • IF Staining of mouse kidney using 28868-1-AP

    IF Staining of mouse kidney using 28868-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using ACE2 antibody (28868-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse kidney using 28868-1-AP

    IF Staining of mouse kidney using 28868-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using ACE2 antibody (28868-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO Validated

ACE2 Polyclonal antibody

ACE2 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 28868-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

Angiotensin-converting enzyme homolog, Angiotensin-converting enzyme 2, Angiotensin converting enzyme 2, ACE-related carboxypeptidase, ACEH

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inmouse kidney tissue, HeLa cells, mouse testis tissue, rat testis tissue
Positive IHC detected inhuman kidney tissue, human colon cancer tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse kidney tissue
Planning an IHC experiment? We recommend our IHCeasy ACE2 Ready-To-Use IHC Kit. ACE2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28868-1-AP targets ACE2 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen ACE2 fusion protein Ag30683 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
Synonyms Angiotensin-converting enzyme homolog, Angiotensin-converting enzyme 2, Angiotensin converting enzyme 2, ACE-related carboxypeptidase, ACEH
Calculated Molecular Weight 805 aa, 92 kDa
Observed Molecular Weight92, 120 kDa
GenBank Accession NumberBC048094
Gene Symbol ACE2
Gene ID (NCBI) 59272
RRIDAB_2881224
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BYF1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACE2 (Angiotensin-converting enzyme 2), also named as ACEH, is a zinc metalloprotease of the ACE family and a critical regulator of the reninangiotensin system. ACE2 has a more restricted tissue distribution than ACE, being found predominantly in the heart, kidneys, and testes although low levels have been detected in a variety of tissues (PMID:15983030). ACE2 has been shown to be a functional receptor of the human coronaviruses SARS-CoV and SARS-CoV-2 (PMID: 32142651). The expression level and expression pattern of human ACE2 in different tissues might be critical for the susceptibility, symptoms, and outcome of 2019-nCoV/SARS-CoV-2 infection (PMID: 32133153). It can be used as a potential therapeutic target of SARS-CoV-2 (PMID: 32125455). The calculated molecular weight of ACE2 is 92kDa but it migrates to 120kDa due to N-glycosylation (PMID:16166094). Sometimes, several cleaved fragments can also be detected as 75kDa, 50 kDa or 37kDa (PMID: 29561187, 22009550, 30759273). It has 2 isoforms produced by alternative splicing. This antibody is specific to ACE2. The location of ACE2 is membrane and cytoplasm, however it accumulates in the nucleus during the mitosis (PMID: 1730413/PMID: 18292088). 28868-1-AP is specific for ACE2, and its antigen region is 30-356aa.

实验方案

Product Specific Protocols
WB protocol for ACE2 antibody 28868-1-APDownload protocol
IHC protocol for ACE2 antibody 28868-1-APDownload protocol
IF protocol for ACE2 antibody 28868-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols