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  • Featured Product
  • KD/KO Validated

ACE2 Monoclonal antibody, PBS Only

ACE2 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F12A4

Cat no : 66699-1-PBS

Print datasheet

Synonyms

Angiotensin-converting enzyme 2, Angiotensin converting enzyme 2, ACE-related carboxypeptidase, ACEH, ACE related carboxypeptidase

验证数据展示

  • WB analysis of human testis using 66699-1-Ig (same clone as 66699-1-PBS)

    WB analysis of human testis using 66699-1-Ig (same clone as 66699-1-PBS)

    Untreated and PNGase F-treated lysates of human testis tissue were subjected to SDS PAGE followed by western blot with 66699-1-Ig (ACE2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808). This data was developed using the same antibody clone with 66699-1-PBS in a different storage buffer formulation.

  • IHC staining of human small intestine using 66699-1-Ig (same clone as 66699-1-PBS)

    IHC staining of human small intestine using 66699-1-Ig (same clone as 66699-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66699-1-Ig (ACE2 antibody) at dilution of 1:2000 (under 10x lens).. This data was developed using the same antibody clone with 66699-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ACE2 antibody included
  • IHC staining of human small intestine using 66699-1-Ig (same clone as 66699-1-PBS)

    IHC staining of human small intestine using 66699-1-Ig (same clone as 66699-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66699-1-Ig (ACE2 antibody) at dilution of 1:2000 (under 40x lens).. This data was developed using the same antibody clone with 66699-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ACE2 antibody included
  • IF Staining of human kidney using 66699-1-Ig (same clone as 66699-1-PBS)

    IF Staining of human kidney using 66699-1-Ig (same clone as 66699-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using ACE2 antibody (66699-1-Ig, Clone: 2F12A4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66699-1-PBS in a different storage buffer formulation.

  • IF Staining of human kidney using 66699-1-Ig (same clone as 66699-1-PBS)

    IF Staining of human kidney using 66699-1-Ig (same clone as 66699-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using ACE2 antibody (66699-1-Ig, Clone: 2F12A4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66699-1-PBS in a different storage buffer formulation.

  • WB analysis of human testis using 66699-1-Ig (same clone as 66699-1-PBS)

    WB analysis of human testis using 66699-1-Ig (same clone as 66699-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 66699-1-Ig (ACE2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66699-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy ACE2 Ready-To-Use IHC Kit. ACE2 primary antibody included.

产品信息

66699-1-PBS targets ACE2 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF-P, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen ACE2 fusion protein Ag15554 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
Synonyms Angiotensin-converting enzyme 2, Angiotensin converting enzyme 2, ACE-related carboxypeptidase, ACEH, ACE related carboxypeptidase
Calculated Molecular Weight 805 aa, 92 kDa
Observed Molecular Weight 120 kDa, 92 kDa
GenBank Accession NumberBC048094
Gene Symbol ACE2
Gene ID (NCBI) 59272
RRIDAB_2882052
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9BYF1
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ACE2 (Angiotensin-converting enzyme 2), also named as ACEH, is a zinc metalloprotease of the ACE family and a critical regulator of the reninangiotensin system. ACE2 has a more restricted tissue distribution than ACE, being found predominantly in the heart, kidneys, and testes although low levels have been detected in a variety of tissues (PMID:15983030). ACE2 has been shown to be a functional receptor of the human coronaviruses SARS-CoV and SARS-CoV-2 (PMID: 32142651). The expression level and expression pattern of human ACE2 in different tissues might be critical for the susceptibility, symptoms, and outcome of 2019-nCoV/SARS-CoV-2 infection (PMID: 32133153). It can be used as a potential therapeutic target of SARS-CoV-2 (PMID: 32125455). The calculated molecular weight of ACE2 is 92kDa but it migrates to 120kDa due to N-glycosylation (PMID:16166094). Sometimes, several cleaved fragments can also be detected as 75kDa, 50 kDa or 37kDa (PMID: 29561187, 22009550, 30759273). It has 2 isoforms produced by alternative splicing. This antibody is specific to ACE2. The location of ACE2 is membrane and cytoplasm, however it accumulates in the nucleus during the mitosis (PMID: 1730413/PMID: 18292088).