• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

ACE2 Recombinant antibody, PBS Only

ACE2 Uni-rAbTM Recombinant Antibody for IHC, IF-P, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4H12

Cat no : 80063-1-PBS

Print datasheet

Synonyms

ACE related carboxypeptidase, ACE2, ACEH, DKFZp434A014, Metalloprotease MPROT15

验证数据展示

  • IHC staining of human kidney using 80063-1-RR (same clone as 80063-1-PBS)

    IHC staining of human kidney using 80063-1-RR (same clone as 80063-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 80063-1-RR (ACE2 antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80063-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ACE2 antibody included
  • IHC staining of human kidney using 80063-1-RR (same clone as 80063-1-PBS)

    IHC staining of human kidney using 80063-1-RR (same clone as 80063-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 80063-1-RR (ACE2 antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80063-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ACE2 antibody included
  • IF Staining of human kidney using 80063-1-RR (same clone as 80063-1-PBS)

    IF Staining of human kidney using 80063-1-RR (same clone as 80063-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using ACE2 antibody (80063-1-RR, Clone: 4H12 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80063-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy ACE2 Ready-To-Use IHC Kit. ACE2 primary antibody included.

产品信息

80063-1-PBS targets ACE2 in IHC, IF-P, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications IHC, IF-P, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen ACE2 fusion protein Ag30683 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
Synonyms ACE related carboxypeptidase, ACE2, ACEH, DKFZp434A014, Metalloprotease MPROT15
Calculated Molecular Weight 805 aa, 92 kDa
GenBank Accession NumberBC048094
Gene Symbol ACE2
Gene ID (NCBI) 59272
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9BYF1
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ACE2 (Angiotensin-converting enzyme 2), also named as ACEH, is a zinc metalloprotease of the ACE family and a critical regulator of the reninangiotensin system. ACE2 has a more restricted tissue distribution than ACE, being found predominantly in the heart, kidneys, and testes although low levels have been detected in a variety of tissues (PMID:15983030). ACE2 has been shown to be a functional receptor of the human coronaviruses SARS-CoV and SARS-CoV-2 (PMID: 32142651). The expression level and expression pattern of human ACE2 in different tissues might be critical for the susceptibility, symptoms, and outcome of 2019-nCoV/SARS-CoV-2 infection (PMID: 32133153). It can be used as a potential therapeutic target of SARS-CoV-2 (PMID: 32125455). The calculated molecular weight of ACE2 is 92kDa but it migrates to 120kDa due to N-glycosylation (PMID:16166094). Sometimes, several cleaved fragments can also be detected as 75kDa, 50 kDa or 37kDa (PMID: 29561187, 22009550, 30759273). It has 2 isoforms produced by alternative splicing. This antibody is specific to ACE2. The location of ACE2 is membrane and cytoplasm, however it accumulates in the nucleus during the mitosis (PMID: 1730413/PMID: 18292088). 80063-1-RR is specific for ACE2, and its antigen region is 30-356aa.