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  • Featured Product
  • KD/KO Validated

Alpha smooth muscle actin Polyclonal antibody

Alpha smooth muscle actin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (7)

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA and More (3)

Conjugate

Unconjugated

Cat no : 14395-1-AP

Print datasheet

Synonyms

ACTA2, Alpha SMA, a-SMA, smooth muscle actin, Alpha actin 2

验证数据展示

  • IF Staining of C2C12 using 14395-1-AP

    IF Staining of C2C12 using 14395-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using smooth muscle actin antibody (14395-1-AP) at dilution of 1:1600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human liver cancer using 14395-1-AP

    IHC staining of human liver cancer using 14395-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:3000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with smooth muscle actin antibody included
  • IHC staining of human liver cancer using 14395-1-AP

    IHC staining of human liver cancer using 14395-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:3000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with smooth muscle actin antibody included
  • IHC staining of human heart using 14395-1-AP

    IHC staining of human heart using 14395-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody at dilution of 1:5000 (under 10x lens).

    Ready-to-use IHC kits available with smooth muscle actin antibody included
  • WB analysis using 14395-1-AP

    WB analysis using 14395-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14395-1-AP (smooth muscle actin antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse liver using 14395-1-AP

    IP experiment of mouse liver using 14395-1-AP

    IP result of anti-smooth muscle actin (IP:14395-1-AP, 3ug; Detection:14395-1-AP 1:1000) with mouse liver tissue lysate 6000ug.

  • WB analysis of A549 using 14395-1-AP

    WB analysis of A549 using 14395-1-AP

    WB result of smooth muscle actin antibody (14395-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-smooth muscle actin transfected A549 cells.

  • IHC staining of human appendicitis using 14395-1-AP

    IHC staining of human appendicitis using 14395-1-AP

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 14395-1-AP (smooth muscle actin antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with smooth muscle actin antibody included
  • IF Staining of mouse heart using 14395-1-AP

    IF Staining of mouse heart using 14395-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 14395-1-AP (smooth muscle actin antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse small intestine using 14395-1-AP

    IF Staining of mouse small intestine using 14395-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using smooth muscle actin antibody (14395-1-AP) at dilution of 1:4000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of C2C12 using 14395-1-AP

    FC experiment of C2C12 using 14395-1-AP

    1x10^6 C2C12 cells were intracellularly stained with 0.4 ug smooth muscle actin Polyclonal antibody (14395-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inmouse colon tissue, human placenta tissue, A549 cells, HUVEC cells, rat colon tissue, mouse heart tissue, rat heart tissue, mouse liver tissue, mouse kidney tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman liver cancer tissue, human heart tissue, human colon tissue, human hysteromyoma tissue, stromal tumor tissue, human appendicitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse heart tissue, mouse small intestine tissue
Positive IF/ICC detected inC2C12 cells, H9C2 cells
Positive FC (Intra) detected inC2C12 cells
Planning an IHC experiment? We recommend our IHCeasy smooth muscle actin Ready-To-Use IHC Kit. smooth muscle actin primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1500-1:6000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:800-1:3200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14395-1-AP targets Alpha smooth muscle actin in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA, competitive binding assay applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, CoIP, competitive binding assay
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, rabbit, canine, monkey, zebrafish, bovine, sheep
Immunogen Alpha smooth muscle actin fusion protein Ag5886 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name actin, alpha 2, smooth muscle, aorta
Synonyms ACTA2, Alpha SMA, a-SMA, smooth muscle actin, Alpha actin 2
Calculated Molecular Weight 377 aa, 42 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC017554
Gene Symbol Alpha smooth muscle actin
Gene ID (NCBI) 59
RRIDAB_2223009
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP62736
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells. This antibody may cross-react with other isoforms of actins.

实验方案

Product Specific Protocols
WB protocol for Alpha smooth muscle actin antibody 14395-1-APDownload protocol
IHC protocol for Alpha smooth muscle actin antibody 14395-1-APDownload protocol
IF protocol for Alpha smooth muscle actin antibody 14395-1-APDownload protocol
IP protocol for Alpha smooth muscle actin antibody 14395-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

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