验证数据展示
经过测试的应用
| Positive WB detected in | mouse colon tissue, mouse kidney tissue, mouse stomach, rat colon, mouse lung, rat lung |
| Positive IHC detected in | mouse heart tissue, human colon tissue, human colon cancer tissue, human heart tissue, human hepatocirrhosis tissue, human liver cancer tissue, mouse colon tissue, mouse liver tissue, rat colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse heart tissue |
Planning an IHC experiment? We recommend our IHCeasy smooth muscle actin Ready-To-Use IHC Kit. smooth muscle actin primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:3000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
55135-1-AP targets Alpha smooth muscle actin specific in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF-P, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, rabbit |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | actin, alpha 2, smooth muscle, aorta |
| 别名 | ACTA2, Alpha SMA, a-SMA, smooth muscle actin, a SMA |
| 计算分子量 | 42 kDa |
| 观测分子量 | 42 kDa |
| GenBank蛋白编号 | NM_001613 |
| 基因名称 | Alpha smooth muscle actin |
| Gene ID (NCBI) | 59 |
| RRID | AB_10949628 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P62736 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ACTA2, also named as a-SMA,AAT6;ACTSA belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Defects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6). The immunogen of this antibody is a specific domain of ACTA2. This antibody stains the aorta only.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Alpha smooth muscle actin specific antibody 55135-1-AP | Download protocol |
| IHC protocol for Alpha smooth muscle actin specific antibody 55135-1-AP | Download protocol |
| IF protocol for Alpha smooth muscle actin specific antibody 55135-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
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ACS Nano Cysteine-Based Redox-Responsive Nanoparticles for Fibroblast-Targeted Drug Delivery in the Treatment of Myocardial Infarction | ||
Cancer Commun (Lond) Glutamine metabolic microenvironment drives M2 macrophage polarization to mediate trastuzumab resistance in HER2-positive gastric cancer | ||
Nat Commun Copper sulfide nanoparticles as a photothermal switch for TRPV1 signaling to attenuate atherosclerosis. | ||
Mol Cancer Extracellular vesicles of carcinoma-associated fibroblasts creates a pre-metastatic niche in the lung through activating fibroblasts. | ||
J Nanobiotechnology Evolocumab loaded Bio-Liposomes for efficient atherosclerosis therapy |
