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Alpha Actin Polyclonal antibody

Alpha Actin Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 11032-1-AP

Print datasheet

Synonyms

ACTC, ACTC1, ACTC1 cardiac muscle 1, Actin, alpha cardiac muscle 1, actin, alpha, cardiac muscle 1, Alpha actin, Alpha cardiac actin, CMD1R, CMH11

验证数据展示

  • WB analysis of mouse heart using 11032-1-AP

    WB analysis of mouse heart using 11032-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 11032-1-AP (alpha Actin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse heart using 11032-1-AP

    IP experiment of mouse heart using 11032-1-AP

    IP result of anti-Alpha Actin (IP:11032-1-AP, 4ug; Detection:11032-1-AP 1:800) with mouse heart tissue lysate 3200ug.

经过测试的应用

Positive WB detected inmouse heart tissue, mouse skeletal muscle tissue, mouse thymus tissue
Positive IP detected inmouse heart tissue
Planning an IHC experiment? We recommend our IHCeasy ACTC1-specific Ready-To-Use IHC Kit. ACTC1-specific primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11032-1-AP targets Alpha Actin in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen Alpha Actin fusion protein Ag1471 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name actin, alpha, cardiac muscle 1
Synonyms ACTC, ACTC1, ACTC1 cardiac muscle 1, Actin, alpha cardiac muscle 1, actin, alpha, cardiac muscle 1, Alpha actin, Alpha cardiac actin, CMD1R, CMH11
Calculated Molecular Weight 377 aa, 42 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC009978
Gene Symbol ACTC1
Gene ID (NCBI) 70
RRIDAB_2242376
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP68032
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Alpha Actin antibody 11032-1-APDownload protocol
IP protocol for Alpha Actin antibody 11032-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

FASEB J

Zbtb20 deficiency causes cardiac contractile dysfunction in mice.

Authors - An-Jing Ren
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humanWB

Int J Biol Sci

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Authors - Yucheng Gao
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humanIHC

Stem Cells Dev

A model of early human embryonic stem cell differentiation reveals inter- and intracellular changes on transition to squamous epithelium.

Authors - Galat Vasiliy V
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humanWB

Onco Targets Ther

Clinical Significance and Oncogenic Activity of GRWD1 Overexpression in the Development of Colon Carcinoma.

Authors - Xin Zhou
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