验证数据展示
经过测试的应用
| Positive WB detected in | mouse lung tissue, rat lung tissue |
| Positive IHC detected in | human colon tissue, human skin cancer tissue, human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy ACTG1 Ready-To-Use IHC Kit. ACTG1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 6 publications below |
| IF | See 1 publications below |
产品信息
11227-1-AP targets Gamma Actin in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | Gamma Actin fusion protein Ag1728 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | actin, gamma 1 |
| 别名 | ACTG1, Actin, cytoplasmic 2, N-terminally processed, Actin, cytoplasmic 2, actin, actg 1 |
| 计算分子量 | 42 kDa |
| 观测分子量 | 42-45 kDa |
| GenBank蛋白编号 | BC009848 |
| 基因名称 | Gamma Actin |
| Gene ID (NCBI) | 71 |
| RRID | AB_2223507 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P63261 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Gamma Actin antibody 11227-1-AP | Download protocol |
| IHC protocol for Gamma Actin antibody 11227-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
iScience A defective mechanosensing pathway affects fibroblast-to-myofibroblast transition in the old male mouse heart | ||
Front Mol Neurosci F-Actin Dysplasia Involved in Organ of Corti Deformity in Gjb2 Knockdown Mouse Model. | ||
Front Neurosci Detergent-insoluble PFN1 inoculation expedites disease onset and progression in PFN1 transgenic rats | ||
FEBS J Icariin ameliorates glycolytic dysfunction in Alzheimer's disease models by activating the Wnt/β-catenin signaling pathway | ||
Ultrasound Q Ultrasound Microbubble-Stimulated miR-145-5p Inhibits Malignant Behaviors of Breast Cancer Cells by Targeting ACTG1 | ||
Cells Interaction of Receptor-Binding Domain of the SARS-CoV-2 Omicron Variant with hACE2 and Actin |