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ARP3/ARP3B Polyclonal antibody
ARP3/ARP3B Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | A431 cells, mouse spleen tissue, human liver tissue, A549 cells, HL-60 cells, HeLa cells, Jurkat cells, K-562 cells, PC-3 cells |
Positive IP detected in | mouse spleen tissue |
Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:16000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
13822-1-AP targets ARP3/ARP3B in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IP, IHC, ELISA Application Description |
Cited Applications | WB, IF, IP |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Immunogen | ARP3/ARP3B fusion protein Ag4922 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | ARP3 actin-related protein 3 homolog (yeast) |
Synonyms | Actin like protein 3, Actin related protein 3, ACTR3, ACTR3B, Arp11, ARP3, ARP3/ARP3B, Arp4 |
Calculated Molecular Weight | 418 aa 47 kDa |
Observed Molecular Weight | 47 kDa |
GenBank Accession Number | BC044590 |
Gene Symbol | ARP3/ACTR3 |
Gene ID (NCBI) | 10096 |
RRID | AB_2221967 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P61158 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for ARP3/ARP3B antibody 13822-1-AP | Download protocol |
IHC protocol for ARP3/ARP3B antibody 13822-1-AP | Download protocol |
IP protocol for ARP3/ARP3B antibody 13822-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
ACS Nano Oxidation of Reduced Graphene Oxide via Cellular Redox Signaling Modulates Actin-Mediated Neurotransmission. | ||
EMBO Rep WDR63 inhibits Arp2/3-dependent actin polymerization and mediates the function of p53 in suppressing metastasis. | ||
Elife Syncytin-mediated open-ended membrane tubular connections facilitate the intercellular transfer of cargos including Cas9 protein | ||
Int J Biol Sci Rictor promotes cell migration and actin polymerization through regulating ABLIM1 phosphorylation in Hepatocellular Carcinoma. |