验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, human skeletal muscle tissue, mouse cerebellum tissue, mouse heart tissue, mouse ovary tissue, rat heart tissue |
| Positive IP detected in | HEK-293 cells |
| Positive IF/ICC detected in | A549 cells, HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
21505-1-AP targets Arp5/ACTR5 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IP, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | Arp5/ACTR5 fusion protein Ag15976 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ARP5 actin-related protein 5 homolog (yeast) |
| 别名 | Actin related protein 5, ACTR5, Arp5, Arp5/ACTR5, hARP5, Sarcoma antigen NY SAR 16 |
| 计算分子量 | 607 aa, 68 kDa |
| 观测分子量 | 68 kDa |
| GenBank蛋白编号 | BC038402 |
| 基因名称 | ARP5/ACTR5 |
| Gene ID (NCBI) | 79913 |
| RRID | AB_10733470 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9H9F9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Arp5 is a member of nuclear actin-related proteins (ARPs) that function as components of the chromatin- remodeling complex. Recently study reported that Arp5 regulates smooth muscle cell differentiation through interaction with myocardin (Myocd). Arp5 has been identified as negative regulator of Myocd, suppressing Myocd activity in the nucleus through tightly binding with Myocd RPEL motifs. Alternative splicing results in two variants of Arp5: a broadly expressed arp5-common, and a smooth muscle restricted arp5-sm. Expression of Arp5-sm protein is strongly suppressed in well-differentiated smooth muscle cells by partial messenger RNA decay and translational suppression. (24567363)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Arp5/ACTR5 antibody 21505-1-AP | Download protocol |
| IF protocol for Arp5/ACTR5 antibody 21505-1-AP | Download protocol |
| IP protocol for Arp5/ACTR5 antibody 21505-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Elife Actin-related protein 5 functions as a novel modulator of MyoD and MyoG in skeletal muscle and in rhabdomyosarcoma.
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