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  • Featured Product
  • KD/KO Validated

Arp5/ACTR5 Polyclonal antibody

Arp5/ACTR5 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 21505-1-AP

Print datasheet

Synonyms

Actin related protein 5, ACTR5, Arp5, Arp5/ACTR5, hARP5, Sarcoma antigen NY SAR 16

验证数据展示

  • WB analysis of mouse cerebellum using 21505-1-AP

    WB analysis of mouse cerebellum using 21505-1-AP

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 21505-1-AP (Arp5/ACTR5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 21505-1-AP

    IP experiment of HEK-293 using 21505-1-AP

    IP result of anti-Arp5/ACTR5 (IP:21505-1-AP, 4ug; Detection:21505-1-AP 1:500) with HEK-293 cells lysate 1120ug.

  • IF Staining of A549 using 21505-1-AP

    IF Staining of A549 using 21505-1-AP

    Immunofluorescent analysis of A549 cells using 21505-1-AP (Arp5/ACTR5 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • WB analysis of HEK-293 using 21505-1-AP

    WB analysis of HEK-293 using 21505-1-AP

    WB result of Arp5/ACTR5 antibody (21505-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Arp5/ACTR5 transfected HEK-293 cells.

  • WB analysis using 21505-1-AP

    WB analysis using 21505-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21505-1-AP (Arp5/ACTR5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHEK-293 cells, human skeletal muscle tissue, mouse cerebellum tissue, mouse heart tissue, mouse ovary tissue, rat heart tissue
Positive IP detected inHEK-293 cells
Positive IF/ICC detected inA549 cells, HepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21505-1-AP targets Arp5/ACTR5 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IP, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen Arp5/ACTR5 fusion protein Ag15976 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ARP5 actin-related protein 5 homolog (yeast)
Synonyms Actin related protein 5, ACTR5, Arp5, Arp5/ACTR5, hARP5, Sarcoma antigen NY SAR 16
Calculated Molecular Weight 607 aa, 68 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC038402
Gene Symbol ARP5/ACTR5
Gene ID (NCBI) 79913
RRIDAB_10733470
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H9F9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Arp5 is a member of nuclear actin-related proteins (ARPs) that function as components of the chromatin- remodeling complex. Recently study reported that Arp5 regulates smooth muscle cell differentiation through interaction with myocardin (Myocd). Arp5 has been identified as negative regulator of Myocd, suppressing Myocd activity in the nucleus through tightly binding with Myocd RPEL motifs. Alternative splicing results in two variants of Arp5: a broadly expressed arp5-common, and a smooth muscle restricted arp5-sm. Expression of Arp5-sm protein is strongly suppressed in well-differentiated smooth muscle cells by partial messenger RNA decay and translational suppression. (24567363)

实验方案

Product Specific Protocols
WB protocol for Arp5/ACTR5 antibody 21505-1-APDownload protocol
IF protocol for Arp5/ACTR5 antibody 21505-1-APDownload protocol
IP protocol for Arp5/ACTR5 antibody 21505-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

EMBO J

LncRNA HAND2-AS1 promotes liver cancer stem cell self-renewal via BMP signaling.

Authors - Yanying Wang
View Article
ratWB,IF

J Cell Biol

Arp5 is a key regulator of myocardin in smooth muscle cells.

Authors - Tsuyoshi Morita
  • KD Validated
View Article
mouseWB,IF,IP

Elife

Actin-related protein 5 functions as a novel modulator of MyoD and MyoG in skeletal muscle and in rhabdomyosarcoma.

Authors - Tsuyoshi Morita
  • KD Validated
View Article
humanWB

Cell Rep

Activation of the CARD8 Inflammasome Requires a Disordered Region.

Authors - Ashley J Chui
View Article