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CoraLite® Plus 488-conjugated AGR2 Monoclonal antibody

AGR2 Monoclonal Antibody for IF/ICC, IF-P

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig

Applications

IF/ICC, IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A8A8

Cat no : CL488-66768

Print datasheet

Synonyms

GOB 4, AGR 2, AG-2, AG2, AG 2

验证数据展示

  • IF Staining of human colon cancer using CL488-66768

    IF Staining of human colon cancer using CL488-66768

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite® Plus 488 AGR2 antibody (CL488-66768, Clone: 1A8A8 ) at dilution of 1:200.

  • IF Staining of HT-29 using CL488-66768

    IF Staining of HT-29 using CL488-66768

    Immunofluorescent analysis of (-20°C Ethanol) fixed HT-29 cells using CoraLite®®488 AGR2 antibody (CL488-66768, Clone: 1A8A8 ) at dilution of 1:200.

经过测试的应用

Positive IF-P detected inhuman colon cancer tissue
Positive IF/ICC detected inHT-29 cells, human colon cancer tissue
Planning an IHC experiment? We recommend our IHCeasy AGR2 Ready-To-Use IHC Kit. AGR2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66768-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66768 targets AGR2 in IF/ICC, IF-P applications and shows reactivity with human, pig samples.

Tested Applications IF/ICC, IF-P Application Description
Tested Reactivity human, pig
Immunogen AGR2 fusion protein Ag2919 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name anterior gradient homolog 2 (Xenopus laevis)
Synonyms GOB 4, AGR 2, AG-2, AG2, AG 2
Calculated Molecular Weight 175 aa, 20 kDa
Observed Molecular Weight 17 kDa
GenBank Accession NumberBC015503
Gene Symbol AGR2
Gene ID (NCBI) 10551
RRIDAB_2919366
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO95994
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AGR2, also named AG2 or HPC8, encodes anterior gradient protein 2 homolog which belongs to the AGR family. It is a secreted protein localized in endoplasmic reticulum. AGR2 plays roles in MUC2 post-transcriptional synthesis,secretion and production of mucus by intestinal cells. AGR2 was significantly elevated in the pancreatic juice from patients with pre-malignant conditions as well as pancreatic cancer compared to control pancreatic juice samples. AGR2 levels in pancreatic juice could potentially be used to aide in assessment of high-risk patients undergoing endoscopic procedures.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 AGR2 antibody CL488-66768Download protocol
Standard Protocols
Click here to view our Standard Protocols