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验证数据展示

  • IF Staining of human colon cancer using CL488-66768

    IF Staining of human colon cancer using CL488-66768

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite® Plus 488 AGR2 antibody (CL488-66768, Clone: 1A8A8 ) at dilution of 1:200.

  • IF Staining of HT-29 using CL488-66768

    IF Staining of HT-29 using CL488-66768

    Immunofluorescent analysis of (-20°C Ethanol) fixed HT-29 cells using CoraLite®®488 AGR2 antibody (CL488-66768, Clone: 1A8A8 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated AGR2 Monoclonal antibody

AGR2 Monoclonal Antibody for IF/ICC, IF-P
Cat No. CL488-66768

产品说明书

CloneNo. 1A8A8

宿主/亚型

Mouse / IgG2b

种属反应性

human, pig

应用

IF/ICC, IF-P

别名

GOB 4, AGR 2, AG-2, AG2, AG 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF-P detected inhuman colon cancer tissue
Positive IF/ICC detected inHT-29 cells, human colon cancer tissue
Planning an IHC experiment? We recommend our IHCeasy AGR2 Ready-To-Use IHC Kit. AGR2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66768-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66768 targets AGR2 in IF/ICC, IF-P applications and shows reactivity with human, pig samples.

经测试应用 IF/ICC, IF-P Application Description
经测试反应性 human, pig
免疫原

CatNo: Ag2919

Product name: Recombinant human AGR2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 17-175 aa of BC015503

Sequence: YTLARDTTVKPGAKKDTKDSRPKLPQTLSRGWGDQLIWTQTYEEALYKSKTSNKPLMIIHHLDECPHSQALKKVFAENKEIQKLAEQFVLLNLVYETTDKHLSPDGQYVPRIMFVDPSLTVRADITGRYSNRLYAYEPADTALLLDNMKKALKLLKTEL

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 anterior gradient homolog 2 (Xenopus laevis)
别名 GOB 4, AGR 2, AG-2, AG2, AG 2
计算分子量 175 aa, 20 kDa
观测分子量 17 kDa
GenBank蛋白编号BC015503
基因名称 AGR2
Gene ID (NCBI) 10551
RRIDAB_2919366
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDO95994
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AGR2, also named AG2 or HPC8, encodes anterior gradient protein 2 homolog which belongs to the AGR family. It is a secreted protein localized in endoplasmic reticulum. AGR2 plays roles in MUC2 post-transcriptional synthesis,secretion and production of mucus by intestinal cells. AGR2 was significantly elevated in the pancreatic juice from patients with pre-malignant conditions as well as pancreatic cancer compared to control pancreatic juice samples. AGR2 levels in pancreatic juice could potentially be used to aide in assessment of high-risk patients undergoing endoscopic procedures.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 AGR2 antibody CL488-66768Download protocol
Standard Protocols
Click here to view our Standard Protocols