验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HT-29 cells |
Planning an IHC experiment? We recommend our IHCeasy AGR2 Ready-To-Use IHC Kit. AGR2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66768-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-66768 targets AGR2 in IF/ICC applications and shows reactivity with Human, Pig samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | Human, Pig |
| 免疫原 | AGR2 fusion protein Ag2919 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | anterior gradient homolog 2 (Xenopus laevis) |
| 别名 | AG 2, AG2, AGR2, GOB 4, HAG 2, HPC8, PDIA17, XAG 2 |
| 计算分子量 | 175 aa, 20 kDa |
| 观测分子量 | 17 kDa |
| GenBank蛋白编号 | BC015503 |
| 基因名称 | AGR2 |
| Gene ID (NCBI) | 10551 |
| RRID | AB_2920033 |
| 偶联类型 | CoraLite®594 Fluorescent Dye |
| 最大激发/发射波长 | 588 nm / 604 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | O95994 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
AGR2, also named AG2 or HPC8, encodes anterior gradient protein 2 homolog which belongs to the AGR family. It is a secreted protein localized in endoplasmic reticulum. AGR2 plays roles in MUC2 post-transcriptional synthesis,secretion and production of mucus by intestinal cells. AGR2 was significantly elevated in the pancreatic juice from patients with pre-malignant conditions as well as pancreatic cancer compared to control pancreatic juice samples. AGR2 levels in pancreatic juice could potentially be used to aide in assessment of high-risk patients undergoing endoscopic procedures.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 AGR2 antibody CL594-66768 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |