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  • Featured Product
  • KD/KO Validated

AKT Monoclonal antibody, PBS Only

AKT Monoclonal Antibody for WB,IP,Indirect ELISA,IHC,IF,FC

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB,IP,Indirect ELISA,IHC,IF,FC

Conjugate

Unconjugated

CloneNo.

2C5D1

Cat No : 60203-2-PBS

Print datasheet

Synonyms

AKT, AKT (pan), AKT pan, AKT1, AKT1/2/3, pan AKT, PKB, PKB ALPHA, PRKBA, Protein kinase B, Proto oncogene c Akt, RAC, RAC ALPHA, RAC PK alpha, total AKT

验证数据展示

  • WB analysis of A549 using 60203-2-Ig (same clone as 60203-2-PBS)

    WB analysis of A549 using 60203-2-Ig (same clone as 60203-2-PBS)

    WB result of AKT antibody (60203-2-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AKT transfected A549 cells. This data was developed using the same antibody clone with 60203-2-PBS in a different storage buffer formulation.

  • WB analysis using 60203-2-Ig (same clone as 60203-2-PBS)

    WB analysis using 60203-2-Ig (same clone as 60203-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60203-2-Ig (AKT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60203-2-PBS in a different storage buffer formulation.

  • FC experiment of Jurkat using 60203-2-Ig (same clone as 60203-2-PBS)

    FC experiment of Jurkat using 60203-2-Ig (same clone as 60203-2-PBS)

    1X10^6 Jurkat cells were intracellularly stained with 0.2 ug Anti-Human AKT (60203-2-Ig, Clone:2C5D1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 60203-2-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 60203-2-Ig (same clone as 60203-2-PBS)

    IHC staining of human breast cancer using 60203-2-Ig (same clone as 60203-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60203-2-Ig (AKT Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60203-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AKT antibody included
  • IF Staining of MCF-7 using 60203-2-Ig (same clone as 60203-2-PBS)

    IF Staining of MCF-7 using 60203-2-Ig (same clone as 60203-2-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using AKT antibody (60203-2-Ig, Clone: 2C5D1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60203-2-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 60203-2-Ig (same clone as 60203-2-PBS)

    IHC staining of human breast cancer using 60203-2-Ig (same clone as 60203-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60203-2-Ig (AKT Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60203-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AKT antibody included
  • IF Staining of HeLa using 60203-2-Ig (same clone as 60203-2-PBS)

    IF Staining of HeLa using 60203-2-Ig (same clone as 60203-2-PBS)

    Immunofluorescent analysis of HeLa cells using 60203-2-Ig (AKT antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L). This data was developed using the same antibody clone with 60203-2-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 60203-2-Ig (same clone as 60203-2-PBS)

    WB analysis of HEK-293 using 60203-2-Ig (same clone as 60203-2-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 60203-2-Ig (AKT Antibody) and 66444-1-Ig(AKT1-phospho-S473 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60203-2-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 60203-2-Ig (same clone as 60203-2-PBS)

    IP experiment of mouse brain using 60203-2-Ig (same clone as 60203-2-PBS)

    IP result of anti-AKT (IP:60203-2-Ig, 5ug; Detection:60203-2-Ig 1:1000) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 60203-2-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy AKT Ready-To-Use IHC Kit. AKT primary antibody included.

产品信息

60203-2-PBS targets AKT in WB, IP, IF, FC, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB,IP,Indirect ELISA,IHC,IF,FC
Tested Reactivity human, mouse, rat
Immunogen AKT fusion protein Ag16695 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name v-akt murine thymoma viral oncogene homolog 1
Synonyms AKT, AKT (pan), AKT pan, AKT1, AKT1/2/3, pan AKT, PKB, PKB ALPHA, PRKBA, Protein kinase B, Proto oncogene c Akt, RAC, RAC ALPHA, RAC PK alpha, total AKT
Calculated Molecular Weight 56 kDa
Observed Molecular Weight 56-62 kDa
GenBank Accession NumberBC000479
Gene Symbol AKT1
Gene ID (NCBI) 207
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP31749
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. This antibody detects all the members of AKT with/without phospho- modification.