验证数据展示
产品信息
60203-2-PBS targets AKT as part of a matched antibody pair:
MP50177-1: 66444-1-PBS capture and 60203-2-PBS detection (validated in Cytometric bead array)
Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
经测试应用 | WB, IHC, IF/ICC, IP, Cytometric bead array, Indirect ELISA Application Description |
经测试反应性 | human, mouse, rat |
免疫原 | AKT fusion protein Ag16695 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | v-akt murine thymoma viral oncogene homolog 1 |
别名 | AKT pan, AKT 1, AKT (pan), AKT1, AKT1/2/3 |
计算分子量 | 56 kDa |
观测分子量 | 56-62 kDa |
GenBank蛋白编号 | BC000479 |
基因名称 | AKT1 |
Gene ID (NCBI) | 207 |
RRID | AB_10912803 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | P31749 |
储存缓冲液 | PBS only , pH 7.3 |
储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. This antibody detects all the members of AKT with/without phospho- modification.