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  • Featured Product
  • KD/KO Validated

AKT Monoclonal antibody, PBS Only (Detector)

AKT Monoclonal Antibody for WB, IHC, IF/ICC, IP, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2C5D1

Cat no : 60203-2-PBS

Print datasheet

Synonyms

AKT pan, AKT 1, AKT (pan), AKT1, AKT1/2/3

验证数据展示

  • WB analysis of A549 using 60203-2-Ig (same clone as 60203-2-PBS)

    WB analysis of A549 using 60203-2-Ig (same clone as 60203-2-PBS)

    WB result of AKT antibody (60203-2-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AKT transfected A549 cells. This data was developed using the same antibody clone with 60203-2-PBS in a different storage buffer formulation.

  • WB analysis using 60203-2-Ig (same clone as 60203-2-PBS)

    WB analysis using 60203-2-Ig (same clone as 60203-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60203-2-Ig (AKT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60203-2-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 60203-2-Ig (same clone as 60203-2-PBS)

    IHC staining of human breast cancer using 60203-2-Ig (same clone as 60203-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60203-2-Ig (AKT Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60203-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AKT antibody included
  • IF Staining of MCF-7 using 60203-2-Ig (same clone as 60203-2-PBS)

    IF Staining of MCF-7 using 60203-2-Ig (same clone as 60203-2-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using AKT antibody (60203-2-Ig, Clone: 2C5D1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60203-2-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 60203-2-Ig (same clone as 60203-2-PBS)

    IHC staining of human breast cancer using 60203-2-Ig (same clone as 60203-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60203-2-Ig (AKT Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60203-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AKT antibody included
  • WB analysis of HEK-293 using 60203-2-Ig (same clone as 60203-2-PBS)

    WB analysis of HEK-293 using 60203-2-Ig (same clone as 60203-2-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 60203-2-Ig (AKT Antibody) and 66444-1-Ig(AKT1-phospho-S473 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60203-2-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 60203-2-Ig (same clone as 60203-2-PBS)

    IP experiment of mouse brain using 60203-2-Ig (same clone as 60203-2-PBS)

    IP result of anti-AKT (IP:60203-2-Ig, 5ug; Detection:60203-2-Ig 1:1000) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 60203-2-PBS in a different storage buffer formulation.

  • Cytometric bead array in cell lysate using MP50177-1

    Cytometric bead array in cell lysate using MP50177-1

    Cytometric bead array in cell lysate using MP50177-1, Phospho-AKT(Ser473) Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66444-1-PBS. Detection antibody: 60203-2-PBS. Cell lysate: Non-treated HEK293 and Calyculin A treated HEK293(30μg/well). Non-related target OAT Monoclonal Matched Antibody Pair (MP50109-1P) was served as control.

Planning an IHC experiment? We recommend our IHCeasy AKT Ready-To-Use IHC Kit. AKT primary antibody included.

产品信息

60203-2-PBS targets AKT as part of a matched antibody pair:

MP50177-1: 66444-1-PBS capture and 60203-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, IF/ICC, IP, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen AKT fusion protein Ag16695 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name v-akt murine thymoma viral oncogene homolog 1
Synonyms AKT pan, AKT 1, AKT (pan), AKT1, AKT1/2/3
Calculated Molecular Weight 56 kDa
Observed Molecular Weight 56-62 kDa
GenBank Accession NumberBC000479
Gene Symbol AKT1
Gene ID (NCBI) 207
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP31749
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. This antibody detects all the members of AKT with/without phospho- modification.