AKT3 Polyclonal antibody
AKT3 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | mouse brain tissue, rat brain tissue |
Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 11 publications below |
IHC | See 1 publications below |
产品信息
21641-1-AP targets AKT3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, ELISA Application Description |
Cited Applications | WB, IHC |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human |
Immunogen | AKT3 fusion protein Ag16298 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma) |
Synonyms | AKT3, PKB GAMMA, PKBG, PRKBG, Protein kinase Akt 3, Protein kinase B gamma, RAC gamma, RAC PK gamma, STK 2 |
Calculated Molecular Weight | 465 aa, 54 kDa |
Observed Molecular Weight | 60 kDa |
GenBank Accession Number | BC121154 |
Gene Symbol | AKT3 |
Gene ID (NCBI) | 10000 |
RRID | AB_10733649 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9Y243 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for AKT3 antibody 21641-1-AP | Download protocol |
IHC protocol for AKT3 antibody 21641-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Front Oncol Long Non-coding RNA AK025387 Promotes Cell Migration and Invasion of Gastric Cancer. | ||
J Cell Mol Med EGR1 interacts with DNMT3L to inhibit the transcription of miR-195 and plays an anti-apoptotic role in the development of gastric cancer. | ||
Clin Epigenetics Alterations of DNA methylation were associated with the rapid growth of cortisol-producing adrenocortical adenoma during pregnancy. | ||
Cancer Cell Int NEK7 promotes gastric cancer progression as a cell proliferation regulator. | ||
Exp Hematol Oncol YTHDF1 promotes hepatocellular carcinoma progression via activating PI3K/AKT/mTOR signaling pathway and inducing epithelial-mesenchymal transition. | ||
Sci Rep Flotillin-2 promotes metastasis of nasopharyngeal carcinoma by activating NF-κB and PI3K/Akt3 signaling pathways. |