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ALDH1A1 Polyclonal antibody
ALDH1A1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA and More (2)
Conjugate
Unconjugated
127
验证数据展示
经过测试的应用
Positive WB detected in | K-562 cells, mouse liver tissue, rat liver tissue, mouse brain tissue, mouse kidney tissue |
Positive IP detected in | mouse liver tissue |
Positive IHC detected in | mouse testis tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse colon tissue, mouse testis tissue |
Positive IF/ICC detected in | A549 cells |
Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
15910-1-AP targets ALDH1A1 in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA Application Description |
Cited Applications | WB, IHC, IF, CoIP, ELISA |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, bovine |
Immunogen | ALDH1A1 fusion protein Ag8665 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | aldehyde dehydrogenase 1 family, member A1 |
Synonyms | ALDH1A 1, ALDH1 A1, ALDH 1A1, Aldehyde dehydrogenase 1A1, ALDC |
Calculated Molecular Weight | 501 aa, 55 kDa |
Observed Molecular Weight | 50-55 kDa |
GenBank Accession Number | BC001505 |
Gene Symbol | ALDH1A1 |
Gene ID (NCBI) | 216 |
RRID | AB_2305276 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P00352 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for ALDH1A1 antibody 15910-1-AP | Download protocol |
IHC protocol for ALDH1A1 antibody 15910-1-AP | Download protocol |
IF protocol for ALDH1A1 antibody 15910-1-AP | Download protocol |
IP protocol for ALDH1A1 antibody 15910-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Res Excessive UBE3A dosage impairs retinoic acid signaling and synaptic plasticity in autism spectrum disorders. | ||
Int J Oral Sci LIMP-2 enhances cancer stem-like cell properties by promoting autophagy-induced GSK3β degradation in head and neck squamous cell carcinoma | ||
Nat Commun TSPAN8 promotes cancer cell stemness via activation of sonic Hedgehog signaling. | ||
J Pineal Res Melatonin inhibits the stemness of head and neck squamous cell carcinoma by modulating HA synthesis via the FOSL1/HAS3 axis | ||
EMBO J RASSF1C oncogene elicits amoeboid invasion, cancer stemness, and extracellular vesicle release via a SRC/Rho axis. |