验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy ALDOA Ready-To-Use IHC Kit. ALDOA primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67453-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-67453 targets ALDOA in IF/ICC applications and shows reactivity with Human, Mouse, Rat samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | Human, Mouse, Rat |
| 免疫原 | ALDOA fusion protein Ag17952 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | aldolase A, fructose-bisphosphate |
| 别名 | ALDA, ALDOA, Lung cancer antigen NY LU 1, Muscle type aldolase |
| 计算分子量 | 39 kDa |
| 观测分子量 | 35-40 kDa |
| GenBank蛋白编号 | BC016800 |
| 基因名称 | ALDOA |
| Gene ID (NCBI) | 226 |
| RRID | AB_2919478 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P04075 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ALDOA(Fructose-bisphosphate aldolase A) is also named as lung cancer antigen NY-LU-1,muscle-type aldolase,ALDA and belongs to the class I fructose-bisphosphate aldolase family.The ALDOA gene encodes a homotetrameric protein(PMID:14615364).It is found in skeletal muscle and red blood cells, and functions in glycolysis, promoting the reversible cleavage of FBP to triose phosphate, G-3-P and DHAP. ALDOA is upregulated in various cancers, including human lung squamous and renal cell carcinomas(PMID:19937139).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 ALDOA antibody CL488-67453 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |