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验证数据展示

  • IF Staining of HeLa using CL488-67453

    IF Staining of HeLa using CL488-67453

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 ALDOA antibody (CL488-67453, Clone: 1D6C2 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated ALDOA Monoclonal antibody

ALDOA Monoclonal Antibody for IF/ICC
Cat No. CL488-67453

产品说明书

CloneNo. 1D6C2

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse, Rat

应用

IF/ICC

别名

ALDA, ALDOA, Lung cancer antigen NY LU 1, Muscle type aldolase

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ALDOA Ready-To-Use IHC Kit. ALDOA primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67453-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67453 targets ALDOA in IF/ICC applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human, Mouse, Rat
免疫原

CatNo: Ag17952

Product name: Recombinant human ALDOA protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 2-364 aa of BC016800

Sequence: PYQYPALTPEQKKELSDIAHRIVAPGKGILAADESTGSIAKRLQSIGTENTEENRRFYRQLLLTADDRVNPCIGGVILFHETLYQKADDGRPFPQVIKSKGGVVGIKVDKGVVPLAGTNGETTTQGLDGLSERCAQYKKDGADFAKWRCVLKIGEHTPSALAIMENANVLARYASICQQNGIVPIVEPEILPDGDHDLKRCQYVTEKVLAAVYKALSDHHIYLEGTLLKPNMVTPGHACTQKFSHEEIAMATVTALRRTVPPAVTGITFLSGGQSEEEASINLNAINKCPLLKPWALTFSYGRALQASALKAWGGKKENLKAAQEEYVKRALANSLACQGKYTPSGQAGAAASESLFVSNHAY

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 aldolase A, fructose-bisphosphate
别名 ALDA, ALDOA, Lung cancer antigen NY LU 1, Muscle type aldolase
计算分子量 39 kDa
观测分子量35-40 kDa
GenBank蛋白编号BC016800
基因名称 ALDOA
Gene ID (NCBI) 226
RRIDAB_2919478
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein G purification
UNIPROT IDP04075
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ALDOA(Fructose-bisphosphate aldolase A) is also named as lung cancer antigen NY-LU-1,muscle-type aldolase,ALDA and belongs to the class I fructose-bisphosphate aldolase family.The ALDOA gene encodes a homotetrameric protein(PMID:14615364).It is found in skeletal muscle and red blood cells, and functions in glycolysis, promoting the reversible cleavage of FBP to triose phosphate, G-3-P and DHAP. ALDOA is upregulated in various cancers, including human lung squamous and renal cell carcinomas(PMID:19937139).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 ALDOA antibody CL488-67453Download protocol
Standard Protocols
Click here to view our Standard Protocols