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CoraLite®594-conjugated ALDOA Monoclonal antibody

ALDOA Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1D6C2

Cat no : CL594-67453

Print datasheet

Synonyms

ALDA, ALDOA, Lung cancer antigen NY LU 1, Muscle type aldolase

验证数据展示

  • IF Staining of HeLa using CL594-67453

    IF Staining of HeLa using CL594-67453

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594 ALDOA antibody (CL594-67453, Clone: 1D6C2 ) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ALDOA Ready-To-Use IHC Kit. ALDOA primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67453-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67453 targets ALDOA in IF/ICC applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen ALDOA fusion protein Ag17952 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name aldolase A, fructose-bisphosphate
Synonyms ALDA, ALDOA, Lung cancer antigen NY LU 1, Muscle type aldolase
Calculated Molecular Weight 39 kDa
Observed Molecular Weight 39 kDa
GenBank Accession NumberBC016800
Gene Symbol ALDOA
Gene ID (NCBI) 226
RRIDAB_2920140
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP04075
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ALDOA(Fructose-bisphosphate aldolase A) is also named as lung cancer antigen NY-LU-1,muscle-type aldolase,ALDA and belongs to the class I fructose-bisphosphate aldolase family.The ALDOA gene encodes a homotetrameric protein(PMID:14615364).It is found in skeletal muscle and red blood cells, and functions in glycolysis, promoting the reversible cleavage of FBP to triose phosphate, G-3-P and DHAP. ALDOA is upregulated in various cancers, including human lung squamous and renal cell carcinomas(PMID:19937139).

实验方案

Product Specific Protocols
IF protocol for CL594 ALDOA antibody CL594-67453Download protocol
Standard Protocols
Click here to view our Standard Protocols