ALK Polyclonal antibody
ALK Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, MCF-7 cells, U-87 MG cells |
| Positive IHC detected in | human lymphoma tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
24184-1-AP targets ALK in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Immunogen | ALK fusion protein Ag21493 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | anaplastic lymphoma receptor tyrosine kinase |
| Synonyms | ALK, ALK tyrosine kinase receptor, Anaplastic lymphoma kinase, CD246, TFG/ALK |
| Calculated Molecular Weight | 1620 aa, 176 kDa |
| Observed Molecular Weight | 180 kDa |
| GenBank Accession Number | NM_004304 |
| Gene Symbol | ALK |
| Gene ID (NCBI) | 238 |
| RRID | AB_2879449 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UM73 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ALK, also named as CD246, is a receptor tyrosine kinase (RTK) that belongs to the protein kinase superfamily. ALK is usually found in the nervous system and appears to play an important role in the normal development and function of the nervous system. ALK was originally identified as part of the NPM (Nucleophosmin)-ALK oncogenic fusion protein, resulting from the (2;5)(p23;q35) translocation that is frequently associated with anaplastic large-cell lymphoma (ALCL). The EML4 (echinoderm microtubule-associated protein-like 4)-ALK fusion protein have been described in non-small-cell lung cancer (NSCLC), this transforming fusion kinase is a promising candidate for a therapeutic target as well as for a diagnostic molecular marker in NSCLC (PMID: 17625570).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ALK antibody 24184-1-AP | Download protocol |
| IHC protocol for ALK antibody 24184-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Oncol Rep DEK promotes the proliferation and invasion of lung cancers and indicates poor prognosis in lung adenocarcinomas. | ||
Antiviral Res Chromatin binding protein HMGN1 promotes HBV cccDNA transcription and replication by regulating the phosphorylation of histone 3 |
