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ALS2 Polyclonal antibody

ALS2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 13998-1-AP

Print datasheet

Synonyms

PLSJ, IAHSP, ALSJ, Alsin, ALS2CR6

验证数据展示

  • WB analysis using 13998-1-AP

    WB analysis using 13998-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13998-1-AP (ALS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 13998-1-AP

    IP experiment of HEK-293 using 13998-1-AP

    IP result of anti-ALS2 (IP:13998-1-AP, 4ug; Detection:13998-1-AP 1:300) with HEK-293 cells lysate 4000ug.

  • IHC staining of human pancreas using 13998-1-AP

    IHC staining of human pancreas using 13998-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 13998-1-AP (ALS2 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human pancreas using 13998-1-AP

    IHC staining of human pancreas using 13998-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 13998-1-AP (ALS2 Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HEK-293 using 13998-1-AP

    IF Staining of HEK-293 using 13998-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 13998-1-AP (ALS2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse cerebellum tissue, HEK-293 cells, rat cerebellum tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13998-1-AP targets ALS2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen ALS2 fusion protein Ag5087 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name amyotrophic lateral sclerosis 2 (juvenile)
Synonyms PLSJ, IAHSP, ALSJ, Alsin, ALS2CR6
Calculated Molecular Weight 1657 aa, 184 kDa
Observed Molecular Weight 185 kDa
GenBank Accession NumberBC029174
Gene Symbol ALS2
Gene ID (NCBI) 57679
RRIDAB_10858476
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96Q42
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ALS2 antibody 13998-1-APDownload protocol
IHC protocol for ALS2 antibody 13998-1-APDownload protocol
IF protocol for ALS2 antibody 13998-1-APDownload protocol
IP protocol for ALS2 antibody 13998-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols