验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, human brain tissue, HT-1080 cells, HepG2 cells, mouse brain tissue |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, MCF-7 cells |
Planning an IHC experiment? We recommend our IHCeasy AMBRA1 Ready-To-Use IHC Kit. AMBRA1 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13762-1-AP targets AMBRA1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Cited Applications | WB, IHC, IF, IP, CoIP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | AMBRA1 fusion protein Ag4735 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | autophagy/beclin-1 regulator 1 |
| Synonyms | WDR94, KIAA1736, DDB1- and CUL4-associated factor 3, DCAF3, Activating molecule in BECN1-regulated autophagy protein 1 |
| Calculated Molecular Weight | 1208 aa, 133 kDa |
| Observed Molecular Weight | 130-135 kDa |
| GenBank Accession Number | BC045609 |
| Gene Symbol | AMBRA1 |
| Gene ID (NCBI) | 55626 |
| RRID | AB_10642007 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9C0C7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
AMBRA1 (Activating molecule in BECN1-regulated autophagy protein 1), also known as WDR94 or KIAA1736, is a 1,298 amino acid protein that contains three WD repeats. AMBRA1 regulates autophagy and development of the nervous system. Involved in autophagy in controlling protein turnover during neuronal development, and in regulating normal cell survival and proliferation. This antibody works well in WB, IHC and IF application.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for AMBRA1 antibody 13762-1-AP | Download protocol |
| IHC protocol for AMBRA1 antibody 13762-1-AP | Download protocol |
| IF protocol for AMBRA1 antibody 13762-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Pharmacol Res miR-103a-3p regulates mitophagy in Parkinson's disease through Parkin/Ambra1 signaling. | ||
Brain Pathol AMBRA1, a novel α-synuclein-binding protein, is implicated in the pathogenesis of multiple system atrophy.
| ||
Front Cell Dev Biol Autophagy-Related Genes and Long Noncoding RNAs Signatures as Predictive Biomarkers for Osteosarcoma Survival. | ||
Int J Biochem Cell Biol Selenoprotein S attenuates high glucose and/or ox-LDL-induced endothelium injury by regulating Akt/mTOR signaling and autophagy. |
