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  • Featured Product
  • KD/KO Validated

Annexin A2 Polyclonal antibody

Annexin A2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 11256-1-AP

Print datasheet

Synonyms

ANXA2, ANX2L4, ANX2, Annexin-2, Annexin II

验证数据展示

  • WB analysis using 11256-1-AP

    WB analysis using 11256-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11256-1-AP (Annexin A2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis using 11256-1-AP

    WB analysis using 11256-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11256-1-AP (Annexin A2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 11256-1-AP

    WB analysis of HEK-293 using 11256-1-AP

    WB result of Annexin A2 (11256-1-AP, 1:1000) with si-Control and si-Annexin A2 transfected HEK293 cells.

  • WB analysis using 11256-1-AP

    WB analysis using 11256-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11256-1-AP (Annexin A2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 11256-1-AP

    IHC staining of human liver using 11256-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11256-1-AP (Annexin A2 Antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with ANXA2 antibody included
  • IHC staining of human liver using 11256-1-AP

    IHC staining of human liver using 11256-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11256-1-AP (Annexin A2 Antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with ANXA2 antibody included
  • FC experiment of K-562 using 11256-1-AP

    FC experiment of K-562 using 11256-1-AP

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human Annexin A2 (11256-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of T-47D using 11256-1-AP

    IF Staining of T-47D using 11256-1-AP

    Immunofluorescent analysis of (4% PFA) fixed T-47D cells using Annexin A2 antibody (11256-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IP experiment of HeLa using 11256-1-AP

    IP experiment of HeLa using 11256-1-AP

    IP result of anti-Annexin A2 (IP:11256-1-AP, 4ug; Detection:11256-1-AP 1:3000) with HeLa cells lysate 2640 ug.

  • IHC staining of mouse kidney using 11256-1-AP

    IHC staining of mouse kidney using 11256-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11256-1-AP (Annexin A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ANXA2 antibody included
  • IHC staining of mouse liver using 11256-1-AP

    IHC staining of mouse liver using 11256-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11256-1-AP (Annexin A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ANXA2 antibody included
  • IHC staining of mouse placenta using 11256-1-AP

    IHC staining of mouse placenta using 11256-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 11256-1-AP (Annexin A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ANXA2 antibody included
  • IHC staining of human bowen disease using 11256-1-AP

    IHC staining of human bowen disease using 11256-1-AP

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 11256-1-AP (Annexin A2 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ANXA2 antibody included
  • IHC staining of human bowen disease using 11256-1-AP

    IHC staining of human bowen disease using 11256-1-AP

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 11256-1-AP (Annexin A2 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ANXA2 antibody included
  • IF Staining of MCF-7 using 11256-1-AP

    IF Staining of MCF-7 using 11256-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Annexin A2 antibody (11256-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, mouse placenta tissue, NIH/3T3 cells, mouse kidney tissue, rat kidney tissue, HepG2 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman liver tissue, human bowen disease, human breast cancer tissue, human hepatocirrhosis tissue, human lung cancer tissue, human oesophagus cancer tissue, human prostate cancer tissue, mouse kidney tissue, mouse liver tissue, mouse placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inT-47D cells, MCF-7 cells, HeLa cells
Positive FC (Intra) detected inK-562 cells
Planning an IHC experiment? We recommend our IHCeasy ANXA2 Ready-To-Use IHC Kit. ANXA2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11256-1-AP targets Annexin A2 in WB, IHC, IF/ICC, FC (Intra), IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP, RIP, ELISA
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, monkey, chicken, bovine
Immunogen Annexin A2 fusion protein Ag1777 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name annexin A2
Synonyms ANXA2, ANX2L4, ANX2, Annexin-2, Annexin II
Calculated Molecular Weight 339 aa, 39 kDa
Observed Molecular Weight 35-40 kDa
GenBank Accession NumberBC016774
Gene Symbol Annexin A2
Gene ID (NCBI) 302
ENSEMBL Gene IDENSG00000182718
RRIDAB_2057311
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP07355
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Annexin A2 (ANXA2), also named Annexin-2 or Lipocortin II, is a Ca2+ binding protein that is up-regulated in virally transformed cell lines and human tumors. This calcium-regulated membrane-binding protein, whose affinity for calcium is greatly enhanced by anionic phospholipids, may cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis. Heat-stress response may also involve Annexin-2.

实验方案

Product Specific Protocols
WB protocol for Annexin A2 antibody 11256-1-APDownload protocol
IHC protocol for Annexin A2 antibody 11256-1-APDownload protocol
IF protocol for Annexin A2 antibody 11256-1-APDownload protocol
IP protocol for Annexin A2 antibody 11256-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human

Nat Immunol

NKILA lncRNA promotes tumor immune evasion by sensitizing T cells to activation-induced cell death.

Authors - Di Huang
View Article
humanWB

Sci Adv

p53 loss activates prometastatic secretory vesicle biogenesis in the Golgi.

Authors - Xiaochao Tan
View Article
humanWB

Nat Chem Biol

YAP-dependent proliferation by a small molecule targeting annexin A2.

Authors - Sophia Z Shalhout
  • KD Validated
View Article
humanWB

Nat Commun

PMID: 31792210

Authors - Sunwang Xu
View Article
mouseWB

Nucleic Acids Res

Short DNA/RNA heteroduplex oligonucleotide interacting proteins are key regulators of target gene silencing.

Authors - Ken Asada
View Article
humanIF,IHC

EBioMedicine

Compounds targeting YadC of uropathogenic Escherichia coli and its host receptor annexin A2 decrease bacterial colonization in bladder.

Authors - Xiao Li
View Article