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验证数据展示

  • ELISA experiment of peptide using 24493-1-AP

    ELISA experiment of peptide using 24493-1-AP

    AP repeat Antibody (24493-1-AP) tested in ELISA. AP repeat peptide was coated onto the microplates at 1.5 µg/well and then incubated with dilution series of AP repeat antibody (24493-1-AP). Bound antibodies were detected with HRP conjugated anti-Rabbit IgG followed by incubation with HRP Substrate and then measuring the resulting absorbance at 450 nm.

AP repeat Polyclonal antibody

AP repeat Polyclonal Antibody for ELISA
Cat No. 24493-1-AP

产品说明书

引用文献(7)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

ELISA and More (3)

别名

AP repeat, APAPAPAPA, C9RAN, GGGCC, poly AP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive ELISA detected inpeptide

推荐稀释比

应用推荐稀释比
Enzyme-linked Immunosorbent Assay (ELISA)ELISA : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24493-1-AP targets AP repeat in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

经测试应用 ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human
文献引用反应性human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 AP repeat
别名 AP repeat, APAPAPAPA, C9RAN, GGGCC, poly AP
基因名称
Gene ID (NCBI)
RRIDAB_2879572
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The C9orf72 "GGGGCC" repeat sequence codes five repeat peptide "GA repeat; GAGAGAGAGA", "GP repeat; GPGPGPGPG", "GR repeat; GRGRGRGRG", "AP repeat; APAPAPAPA" and "PR repeat; PRPRPRPRP". It was described previously that aggregated forms of poly-GA and poly-GP proteins do not enter the separation gel (PMID: 26374446). This antibody is used to detect the "AP repeat" sequence. This antibody detects the APAPAPAPA peptide with dilution 1:32,000 in Elisa.

发表文章

SpeciesApplicationTitle
humanIHC

Acta Neuropathol

Increased prevalence of granulovacuolar degeneration in C9orf72 mutation.

Authors - Yuichi Riku
View Article
humanWB, IHC

Acta Neuropathol

Quantitative analysis and clinico-pathological correlations of different dipeptide repeat protein pathologies in C9ORF72 mutation carriers.

Authors - Ian R A Mackenzie
View Article
humanIF

Acta Neuropathol

Antisense RNA foci in the motor neurons of C9ORF72-ALS patients are associated with TDP-43 proteinopathy.

Authors - Johnathan Cooper-Knock
View Article
humanWB,IF

Hum Mol Genet

Modelling C9orf72 dipeptide repeat proteins of a physiologically relevant size.

Authors - Janis Bennion Callister
View Article
humanWB

J Cell Sci

SFPQ regulates the accumulation of RNA foci and dipeptide repeat proteins from the expanded repeat mutation in C9orf72.

Authors - Mirjana Malnar
View Article
humanWB

Life Sci Alliance

C9ORF72-derived poly-GA DPRs undergo endocytic uptake in iAstrocytes and spread to motor neurons.

Authors - Paolo M Marchi
View Article