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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • ELISA experiment of peptide using 24493-1-AP

    ELISA experiment of peptide using 24493-1-AP

    AP repeat Antibody (24493-1-AP) tested in ELISA. AP repeat peptide was coated onto the microplates at 1.5 µg/well and then incubated with dilution series of AP repeat antibody (24493-1-AP). Bound antibodies were detected with HRP conjugated anti-Rabbit IgG followed by incubation with HRP Substrate and then measuring the resulting absorbance at 450 nm.

AP repeat Polyclonal antibody

AP repeat Polyclonal Antibody for ELISA
Cat No. 24493-1-AP

Print datasheet

Publications(7)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

ELISA and More (3)

Synonyms

AP repeat, APAPAPAPA, C9RAN, GGGCC, poly AP

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive ELISA detected inpeptide

推荐稀释比

ApplicationDilution
Enzyme-linked Immunosorbent Assay (ELISA)ELISA : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24493-1-AP targets AP repeat in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Applications ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name AP repeat
Synonyms AP repeat, APAPAPAPA, C9RAN, GGGCC, poly AP
Gene Symbol
Gene ID (NCBI)
RRIDAB_2879572
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The C9orf72 "GGGGCC" repeat sequence codes five repeat peptide "GA repeat; GAGAGAGAGA", "GP repeat; GPGPGPGPG", "GR repeat; GRGRGRGRG", "AP repeat; APAPAPAPA" and "PR repeat; PRPRPRPRP". It was described previously that aggregated forms of poly-GA and poly-GP proteins do not enter the separation gel (PMID: 26374446). This antibody is used to detect the "AP repeat" sequence. This antibody detects the APAPAPAPA peptide with dilution 1:32,000 in Elisa.

发表文章

SpeciesApplicationTitle
humanIHC

Acta Neuropathol

Increased prevalence of granulovacuolar degeneration in C9orf72 mutation.

Authors - Yuichi Riku
View Article
humanWB, IHC

Acta Neuropathol

Quantitative analysis and clinico-pathological correlations of different dipeptide repeat protein pathologies in C9ORF72 mutation carriers.

Authors - Ian R A Mackenzie
View Article
humanIF

Acta Neuropathol

Antisense RNA foci in the motor neurons of C9ORF72-ALS patients are associated with TDP-43 proteinopathy.

Authors - Johnathan Cooper-Knock
View Article
humanWB,IF

Hum Mol Genet

Modelling C9orf72 dipeptide repeat proteins of a physiologically relevant size.

Authors - Janis Bennion Callister
View Article
humanWB

J Cell Sci

SFPQ regulates the accumulation of RNA foci and dipeptide repeat proteins from the expanded repeat mutation in C9orf72.

Authors - Mirjana Malnar
View Article
humanWB

Life Sci Alliance

C9ORF72-derived poly-GA DPRs undergo endocytic uptake in iAstrocytes and spread to motor neurons.

Authors - Paolo M Marchi
View Article