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APOBEC3G Monoclonal antibody

APOBEC3G Monoclonal Antibody for WB, IF, IHC, ELISA
Cat No. 60100-1-Ig

产品说明书

CloneNo. 5G10A8

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

WB, IF, IHC, ELISA

APOBEC related protein, APOBEC related protein 9, APOBEC3G, ARCD, ARP 9, ARP9, bK150C2.7, CEM 15, CEM15, MDS019

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inA375 cells, PC-3 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inC6 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60100-1-Ig targets APOBEC3G in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF, IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 APOBEC3G fusion protein Ag7770 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3G
别名 APOBEC related protein, APOBEC related protein 9, APOBEC3G, ARCD, ARP 9, ARP9, bK150C2.7, CEM 15, CEM15, MDS019
计算分子量 46 kDa
观测分子量 46 kDa
GenBank蛋白编号BC024268
基因名称 APOBEC3G
Gene ID (NCBI) 60489
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9HC16
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

APOBEC3G((apolipoprotein B mRNA-editing enzyme, catalytic polypeptide-like 3G,also known as CEM15), a member of the APOBEC family of cytidine deaminase-editing enzymes, is a potent DNA mutator that can be packaged into budding retroviruses.HA-tagged APOBEC3G can be expressed in 293T cells, which do not express APOBEC3G endogenously,in addition 22- and 24-kDa proteolytic degradation fragments can be detected by western blot(PMID:14672928). It has 2 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for APOBEC3G antibody 60100-1-IgDownload protocol
IHC protocol for APOBEC3G antibody 60100-1-IgDownload protocol
IF protocol for APOBEC3G antibody 60100-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Sci Rep

Hepatitis B virus X protein is capable of down-regulating protein level of host antiviral protein APOBEC3G.

Authors - Ruidong Chen
humanIHC

Int J Clin Exp Med

APOBEC3G expression is correlated with poor prognosis in colon carcinoma patients with hepatic metastasis.

Authors - Huanrong Lan
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