• Featured Product
  • KD/KO Validated

ARNT/HIF-1 beta Polyclonal antibody

ARNT/HIF-1 beta Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 14105-1-AP

Print datasheet

Synonyms

ARNT, HIF1B, HIF1 beta, HIF 1 beta, Class E basic helix-loop-helix protein 2



经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells
Positive IP detected inHepG2 cells
Positive IHC detected inmouse embryo tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy ARNT Ready-To-Use IHC Kit. ARNT primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14105-1-AP targets ARNT/HIF-1 beta in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Immunogen ARNT/HIF-1 beta fusion protein Ag5238 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name aryl hydrocarbon receptor nuclear translocator
Synonyms ARNT, HIF1B, HIF1 beta, HIF 1 beta, Class E basic helix-loop-helix protein 2
Calculated Molecular Weight 416 aa, 46 kDa, 87 kDa
Observed Molecular Weight 86 kDa
GenBank Accession NumberBC060838
Gene Symbol ARNT/HIF-1 beta
Gene ID (NCBI) 405
RRIDAB_10639523
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP27540
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HIF1B(HIF-1 beta), also named as ARNT, is a 789 amino acid protein, which forms a heterodimer with AHR, AHRR, HIF1A and EPAS1/HIF2A as well as with other bHLH proteins. HIF1B is Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia

实验方案

Product Specific Protocols
WB protocol for ARNT/HIF-1 beta antibody 14105-1-APDownload protocol
IHC protocol for ARNT/HIF-1 beta antibody 14105-1-APDownload protocol
IF protocol for ARNT/HIF-1 beta antibody 14105-1-APDownload protocol
IP protocol for ARNT/HIF-1 beta antibody 14105-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Hepatology

PROX1 promotes hepatocellular carcinoma metastasis by way of up-regulating hypoxia-inducible factor 1α expression and protein stability.

Authors - Liu Yanfeng Y
humanWB,IF

BMC Med

NUPR1 contributes to radiation resistance by maintaining ROS homeostasis via AhR/CYP signal axis in hepatocellular carcinoma

Authors - Yizhi Zhan
humanWB

Free Radic Biol Med

Sestrin2 inhibits hypoxia-inducible factor-1α accumulation via AMPK-mediated prolyl hydroxylase regulation.

Authors - Kyuhwa Seo
mouseWB

Cell Immunol

Fine particulate matter (PM2.5) induces inhibitory memory alveolar macrophages through the AhR/IL-33 pathway

Authors - Yanan Liu
mouseWB

Front Microbiol

Fine Particulate Matter (PM2.5) Promotes CD146 Expression in Alveolar Epithelial Cells and Cryptococcus neoformans Pulmonary Infection.

Authors - Zhixiao Sun
mouseWB

J Biomed Nanotechnol

Fine Particulate Matter (PM2.5) Promoted the Invasion of Lung Cancer Cells via an ARNT2/PP2A/STAT3/MMP2 Pathway.

Authors - Zhongqi Chen