- Featured Product
- KD/KO Validated
ARNT/HIF-1 beta Polyclonal antibody
ARNT/HIF-1 beta Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IHC, IF/ICC, IP, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, HeLa cells, HepG2 cells |
Positive IP detected in | HepG2 cells |
Positive IHC detected in | mouse embryo tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
14105-1-AP targets ARNT/HIF-1 beta in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
Cited Applications | WB, IHC, IF, IP |
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse, rat |
Immunogen | ARNT/HIF-1 beta fusion protein Ag5238 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | aryl hydrocarbon receptor nuclear translocator |
Synonyms | ARNT, HIF1B, HIF1 beta, HIF 1 beta, Class E basic helix-loop-helix protein 2 |
Calculated Molecular Weight | 416 aa, 46 kDa, 87 kDa |
Observed Molecular Weight | 86 kDa |
GenBank Accession Number | BC060838 |
Gene Symbol | ARNT/HIF-1 beta |
Gene ID (NCBI) | 405 |
RRID | AB_10639523 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P27540 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for ARNT/HIF-1 beta antibody 14105-1-AP | Download protocol |
IHC protocol for ARNT/HIF-1 beta antibody 14105-1-AP | Download protocol |
IF protocol for ARNT/HIF-1 beta antibody 14105-1-AP | Download protocol |
IP protocol for ARNT/HIF-1 beta antibody 14105-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Hepatology PROX1 promotes hepatocellular carcinoma metastasis by way of up-regulating hypoxia-inducible factor 1α expression and protein stability. | ||
BMC Med NUPR1 contributes to radiation resistance by maintaining ROS homeostasis via AhR/CYP signal axis in hepatocellular carcinoma | ||
Free Radic Biol Med Sestrin2 inhibits hypoxia-inducible factor-1α accumulation via AMPK-mediated prolyl hydroxylase regulation. | ||
Cell Immunol Fine particulate matter (PM2.5) induces inhibitory memory alveolar macrophages through the AhR/IL-33 pathway | ||
Front Microbiol Fine Particulate Matter (PM2.5) Promotes CD146 Expression in Alveolar Epithelial Cells and Cryptococcus neoformans Pulmonary Infection. | ||
J Biomed Nanotechnol Fine Particulate Matter (PM2.5) Promoted the Invasion of Lung Cancer Cells via an ARNT2/PP2A/STAT3/MMP2 Pathway. |