验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells, Jurkat cells, Y79 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
产品信息
12301-1-AP targets ASCC1 in WB, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Immunogen | ASCC1 fusion protein Ag2946 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | activating signal cointegrator 1 complex subunit 1 |
| Synonyms | SMABF2, CGI18, CGI 18, ASCC 1, ASC1p50 |
| Calculated Molecular Weight | 45.5 kDa |
| Observed Molecular Weight | 45.5 kDa |
| GenBank Accession Number | BC012291 |
| Gene Symbol | ASCC1 |
| Gene ID (NCBI) | 51008 |
| RRID | AB_2059350 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ASCC1 (also known as p50) is a subunit of an uncharacterized complex, the activating signal co-integrator complex (ASCC). ASCC1 has been reported as regulator fo NF-kB pathway and potently inhibits the expression of NF-kB target genes. Mutations in ASCC1 in patients with Barrett esophagus (BE) have been identified as a risk factor of esophageal adenocarcinoma (EAC).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ASCC1 antibody 12301-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Extended DNA threading through a dual-engine motor module of the activating signal co-integrator 1 complex | ||
Mol Cell Unresolved stalled ribosome complexes restrict cell-cycle progression after genotoxic stress. |