验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, MCF-7 cells, Raji cells |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
23226-1-AP targets ATG2A in WB, IHC, IF/ICC, IP, CoIP, ELISA, Peptide array applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, CoIP, Peptide array |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | ATG2A fusion protein Ag19709 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ATG2 autophagy related 2 homolog A (S. cerevisiae) |
| 别名 | KIAA0404, Autophagy-related protein 2 homolog A |
| 计算分子量 | 1938 aa, 213 kDa |
| 观测分子量 | 215 kDa |
| GenBank蛋白编号 | BC110650 |
| 基因名称 | ATG2A |
| Gene ID (NCBI) | 23130 |
| RRID | AB_2879235 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen Affinity purified |
| UNIPROT ID | Q2TAZ0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Autophagy-related protein 2 homolog A (ATG2A) belongs to the ATG2 family. ATG2A is a lipid transfer protein involved in autophagosome assembly (PMID:31271352). ATG2A tethers the edge of the isolation membrane (IM) to the endoplasmic reticulum (ER) and mediates direct lipid transfer from ER to IM for IM expansion. ATG2A binds to the ER exit site (ERES), which is the membrane source for autophagosome formation, and extracts phospholipids from the membrane source and transfers them to ATG9 (ATG9A or ATG9B) to the IM for membrane expansion (PMID: 30952800, PMID: 31271352). ATG2A also regulates lipid droplet morphology and distribution within the cell (PMID: 28561066). The observed MW of ATG2A is 215 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ATG2A antibody 23226-1-AP | Download protocol |
| IHC protocol for ATG2A antibody 23226-1-AP | Download protocol |
| IF protocol for ATG2A antibody 23226-1-AP | Download protocol |
| IP protocol for ATG2A antibody 23226-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Cell Biol ATG9A protects the plasma membrane from programmed and incidental permeabilization.
| ||
Mol Cell ATG9A and ATG2A form a heteromeric complex essential for autophagosome formation | ||
Nat Commun The autophagy protein ATG9A enables lipid mobilization from lipid droplets.
| ||
EMBO Rep A conserved ATG2-GABARAP family interaction is critical for phagophore formation. | ||
Theranostics The c-Myc/miR-27b-3p/ATG10 regulatory axis regulates chemoresistance in colorectal cancer. |