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- KD/KO Validated
ATG2B Polyclonal antibody
ATG2B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IHC, IF/ICC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells |
| Positive IHC detected in | human pancreas cancer tissue, human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
25155-1-AP targets ATG2B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Cited Applications | WB, IF |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Immunogen | ATG2B fusion protein Ag18412 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | ATG2 autophagy related 2 homolog B (S. cerevisiae) |
| Synonyms | C14orf103, Autophagy-related protein 2 homolog B |
| Calculated Molecular Weight | 2078 aa, 233 kDa |
| Observed Molecular Weight | 240-250 kDa |
| GenBank Accession Number | BC015016 |
| Gene Symbol | ATG2B |
| Gene ID (NCBI) | 55102 |
| RRID | AB_2810874 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96BY7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ATG2B antibody 25155-1-AP | Download protocol |
| IHC protocol for ATG2B antibody 25155-1-AP | Download protocol |
| IF protocol for ATG2B antibody 25155-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Cell Biol ATG9A protects the plasma membrane from programmed and incidental permeabilization.
| ||
Nat Commun The autophagy protein ATG9A enables lipid mobilization from lipid droplets.
| ||
Autophagy SHISA5/SCOTIN restrains spontaneous autophagy induction by blocking contact between the ERES and phagophores. | ||
Autophagy Architecture of the ATG2B-WDR45 complex and an aromatic Y/HF motif crucial for complex formation. | ||
EMBO Rep A conserved ATG2-GABARAP family interaction is critical for phagophore formation. |
