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ATG5 Polyclonal antibody

ATG5 Polyclonal Antibody for WB, IP, ELISA
Cat No. 10181-2-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (7)

应用

WB, IP, ELISA and More (3)

Apoptosis-specific protein, Apoptosis specific protein, APG5-like, APG5L, APG5 LIKE

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inA549 cells, HeLa cells, mouse kidney tissue, HepG2 cells
Positive IP detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10181-2-AP targets ATG5 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, pig, monkey, chicken, bovine, hamster, goat, grouper
免疫原 ATG5 fusion protein Ag0214 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ATG5 autophagy related 5 homolog (S. cerevisiae)
别名 Apoptosis-specific protein, Apoptosis specific protein, APG5-like, APG5L, APG5 LIKE
计算分子量 32 kDa
观测分子量 32 kDa, 40-45 kDa, 50-55 kDa
GenBank蛋白编号BC002699
基因名称 ATG5
Gene ID (NCBI) 9474
RRIDAB_2062045
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H1Y0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATG5, also named as APG5L and ASP, belongs to the ATG5 family. It is required for autophagy. It plays an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles. It mediates autophagosome-independent host protection. This antibody is raised against 28-275 amino acids of human ATG5. It can recognize the ATG5-ATG12 complex (55 kDa) which can be truncated and generate a 40-45 kDa band. 10181-2-AP also recognizes the free ATG5 (32 kDa).

实验方案

Product Specific Protocols
WB protocol for ATG5 antibody 10181-2-APDownload protocol
IHC protocol for ATG5 antibody 10181-2-APDownload protocol
IP protocol for ATG5 antibody 10181-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Signal Transduct Target Ther

Targeting CRL4 suppresses chemoresistant ovarian cancer growth by inducing mitophagy

Authors - Yang Meng
humanWB

Cell Res

Degradation of lipid droplets by chimeric autophagy-tethering compounds.

Authors - Yuhua Fu
  • KO Validated
humanWB

Mol Cell

Syntaxin 13, a Genetic Modifier of Mutant CHMP2B in Frontotemporal Dementia, Is Required for Autophagosome Maturation.

Authors - Lu Yubing Y
  • KD Validated
humanWB

Adv Sci (Weinh)

SETDB1 Methylates MCT1 Promoting Tumor Progression by Enhancing the Lactate Shuttle

Authors - Xiaowei She
human

Autophagy

Autophagy mediates the clearance of oligodendroglial SNCA/alpha-synuclein and TPPP/p25A in multiple system atrophy models

Authors - Panagiota Mavroeidi
mouseWB

J Clin Invest

Acetaldehyde dehydrogenase 2 interactions with LDLR and AMPK regulate foam cell formation.

Authors - Shanshan Zhong
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