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- KD/KO Validated
ATG5 Polyclonal antibody
ATG5 Polyclonal Antibody for WB, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (7)
Applications
WB, IP, ELISA and More (2)
Conjugate
Unconjugated
323
验证数据展示
经过测试的应用
Positive WB detected in | A549 cells, HeLa cells, mouse kidney tissue, HepG2 cells |
Positive IP detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:5000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
10181-2-AP targets ATG5 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IP, ELISA Application Description |
Cited Applications | WB, IHC, IF, IP |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, pig, monkey, chicken, bovine, hamster, goat, grouper |
Immunogen | ATG5 fusion protein Ag0214 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | ATG5 autophagy related 5 homolog (S. cerevisiae) |
Synonyms | Apoptosis-specific protein, Apoptosis specific protein, APG5-like, APG5L, APG5 LIKE |
Calculated Molecular Weight | 32 kDa |
Observed Molecular Weight | 32 kDa, 40-45 kDa, 50-55 kDa |
GenBank Accession Number | BC002699 |
Gene Symbol | ATG5 |
Gene ID (NCBI) | 9474 |
RRID | AB_2062045 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9H1Y0 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for ATG5 antibody 10181-2-AP | Download protocol |
IHC protocol for ATG5 antibody 10181-2-AP | Download protocol |
IP protocol for ATG5 antibody 10181-2-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Signal Transduct Target Ther Targeting CRL4 suppresses chemoresistant ovarian cancer growth by inducing mitophagy | ||
Cell Res Degradation of lipid droplets by chimeric autophagy-tethering compounds.
| ||
Mol Cell Syntaxin 13, a Genetic Modifier of Mutant CHMP2B in Frontotemporal Dementia, Is Required for Autophagosome Maturation.
| ||
Adv Sci (Weinh) SETDB1 Methylates MCT1 Promoting Tumor Progression by Enhancing the Lactate Shuttle | ||
Autophagy Autophagy mediates the clearance of oligodendroglial SNCA/alpha-synuclein and TPPP/p25A in multiple system atrophy models | ||
J Clin Invest Acetaldehyde dehydrogenase 2 interactions with LDLR and AMPK regulate foam cell formation. |