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ATM Polyclonal antibody

ATM Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 27156-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, IP, ELISA and More (2)

Serine-protein kinase ATM, EC:2.7.11.1, A-T mutated, A T mutated

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman breast cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ATM Ready-To-Use IHC Kit. ATM primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27156-1-AP targets ATM in WB, IHC, IF, IP, ChIP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, ChIP
经测试反应性 human
文献引用反应性human, mouse
免疫原 ATM fusion protein Ag25909 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ataxia telangiectasia mutated
别名 Serine-protein kinase ATM, EC:2.7.11.1, A-T mutated, A T mutated
观测分子量 310-350 kDa
GenBank蛋白编号BC137169
基因名称 ATM
Gene ID (NCBI) 472
RRIDAB_2880780
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13315
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The ATM protein is a member of the phosphatidylinositol 3-kinase family of proteins that respond to DNA damage by phosphorylating key substrates involved in DNA repair and/or cell cycle control. It is involved in mitogenic signal transduction, meiotic recombination, detection of DNA damage, and cell cycle control.

实验方案

Product Specific Protocols
WB protocol for ATM antibody 27156-1-APDownload protocol
IHC protocol for ATM antibody 27156-1-APDownload protocol
IP protocol for ATM antibody 27156-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Sci Transl Med

Guanosine diphosphate-mannose suppresses homologous recombination repair and potentiates antitumor immunity in triple-negative breast cancer

Authors - Jia-Han Ding
mouseWB

Acta Pharm Sin B

Honokiol alleviated neurodegeneration by reducing oxidative stress and improving mitochondrial function in mutant SOD1 cellular and mouse models of amyotrophic lateral sclerosis

Authors - Yujun Zhou
humanWB

Cell Death Differ

p53-dependent elimination of aneuploid mitotic offspring by entosis.

Authors - Jianqing Liang
humanWB

Oncogene

TRIM24 is critical for the cellular response to DNA double-strand breaks through regulating the recruitment of MRN complex

Authors - Ya Wang
WB

Clin Transl Med

Single-cell profiling-guided combination therapy of c-Fos and histone deacetylase inhibitors in diffuse large B-cell lymphoma.

Authors - Luqiao Wang
humanWB

EBioMedicine

MAP4K1 functions as a tumor promotor and drug mediator for AML via modulation of DNA damage/repair system and MAPK pathway.

Authors - Qing Ling
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