验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, A431 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13231-1-AP targets ATP12A in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag4105 Product name: Recombinant human ATP12A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 367-700 aa of BC031609 Sequence: LTAKRMAKKNCLVKNLEAVETLGSTSIICSDKTGTLTQNRMTVAHLWFDNQIFVADTSEDHSNQVFDQSSRTWASLSKIITLCNRAEFKPGQENVPIMKKAVIGDASETALLKFSEVILGDVMEIRKRNRKVAEIPFNSTNKFQLSIHEMDDPHGKRFLMVMKGAPERILEKCSTIMINGEEHPLDKSTAKTFHTAYMELGGLGERVLGFCHLYLPADEFPETYSFDIDAMNFPTSNLCFVGLLSMIDPPRSTVPDAVTKCRSAGIKVIMVTGDHPITAKAIAKSVGIISANSETVEDIAHRLNIAVEQVNKRDAKAAVVTGMELKDMSSEQLD 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ATPase, H+/K+ transporting, nongastric, alpha polypeptide |
| 别名 | ATP12A, ATP1AL1, Proton pump |
| 计算分子量 | 1045 aa, 116 kDa |
| 观测分子量 | 100-110 kDa |
| GenBank蛋白编号 | BC031609 |
| 基因名称 | ATP12A |
| Gene ID (NCBI) | 479 |
| RRID | AB_2877926 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P54707 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ATP12A, also named as ATP1AL1 and Proton pump, belongs to the family of P-type cation transport ATPases. It catalyzes the hydrolysis of ATP coupled with the exchange of H+ and K+ ions across the plasma membrane. ATP12A is responsible for potassium absorption in various tissues. Expression of ATP12A is increased in benign prostate hyperplasia and tumor tissues.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ATP12A antibody 13231-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
