验证数据展示
经过测试的应用
| Positive IHC detected in | mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
Biotin-55179 targets ATP1A2-Specific in IHC applications and shows reactivity with human, mouse samples.
| Tested Applications | IHC Application Description |
| Tested Reactivity | human, mouse |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | ATPase, Na+/K+ transporting, alpha 2 (+) polypeptide |
| Synonyms | ATP1A2, Sodium pump subunit alpha-2, Na(+)/K(+) ATPase alpha-2 subunit, EC:7.2.2.13, ATP1A 2 |
| Calculated Molecular Weight | 112 kDa |
| Observed Molecular Weight | 100 kDa |
| GenBank Accession Number | NM_000702 |
| Gene Symbol | ATP1A2 |
| Gene ID (NCBI) | 477 |
| Conjugate | Biotin Plus |
| Form | Liquid |
| Purification Method | Antigen Affinity Purified |
| UNIPROT ID | P50993 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ATP1A2, also named as KIAA0778, belongs to the cation transport ATPase (P-type) family and Type IIC subfamily. It is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. Defects in ATP1A2 are the cause of familial hemiplegic migraine type 2 (FHM2). Defects in ATP1A2 are a cause of alternating hemiplegia of childhood (AHC). This antibody is specific to ATP1A2.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for Biotin Plus ATP1A2-Specific antibody Biotin-55179 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |