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  • Featured Product
  • KD/KO Validated

ATP1A2-Specific Polyclonal antibody

ATP1A2-Specific Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 55179-1-AP

Print datasheet

Synonyms

ATP1A2, ATP1A2-Specific, FHM2, KIAA0778, MHP2, Sodium pump subunit alpha 2

验证数据展示

  • WB analysis of mouse brain using 55179-1-AP

    WB analysis of mouse brain using 55179-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 55179-1-AP (ATP1A2-Specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human skeletal muscle using 55179-1-AP

    IHC staining of human skeletal muscle using 55179-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55179-1-AP (ATP1A2-Specific antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human skeletal muscle using 55179-1-AP

    IHC staining of human skeletal muscle using 55179-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55179-1-AP (ATP1A2-Specific antibody) at dilution of 1:50 (under 40x lens).

  • IP experiment of mouse brain using 55179-1-AP

    IP experiment of mouse brain using 55179-1-AP

    IP result of anti-ATP1A2-Specific (IP:55179-1-AP, 4ug; Detection:55179-1-AP 1:800) with mouse brain tissue lysate 4000ug.

经过测试的应用

Positive WB detected inmouse brain tissue, human brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman skeletal muscle tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55179-1-AP targets ATP1A2-Specific in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse
Cited Reactivitymouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ATPase, Na+/K+ transporting, alpha 2 (+) polypeptide
Synonyms ATP1A2, ATP1A2-Specific, FHM2, KIAA0778, MHP2, Sodium pump subunit alpha 2
Calculated Molecular Weight 112 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberNM_000702
Gene Symbol ATP1A2
Gene ID (NCBI) 477
RRIDAB_2881284
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP50993
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATP1A2, also named as KIAA0778, belongs to the cation transport ATPase (P-type) family and Type IIC subfamily. It is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. Defects in ATP1A2 are the cause of familial hemiplegic migraine type 2 (FHM2). Defects in ATP1A2 are a cause of alternating hemiplegia of childhood (AHC). This antibody is specific to ATP1A2.

实验方案

Product Specific Protocols
WB protocol for ATP1A2-Specific antibody 55179-1-APDownload protocol
IHC protocol for ATP1A2-Specific antibody 55179-1-APDownload protocol
IP protocol for ATP1A2-Specific antibody 55179-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF

Cell Calcium

α2 isoform of Na+,K+-ATPase via Na+,Ca2+ exchanger modulates myelin basic protein synthesis in oligodendrocyte lineage cells in vitro.

Authors - Jens Hammann
  • KD Validated
View Article
ratWB

Cell Calcium

Na+/Ca2+ exchanger isoform 1 (NCX1) and canonical transient receptor potential channel 6 (TRPC6) are recruited by STIM1 to mediate Store-Operated Calcium Entry in primary cortical neurons.

Authors - Valentina Tedeschi
View Article