验证数据展示
经过测试的应用
| Positive WB detected in | mouse heart tissue, K-562 cells |
| Positive IP detected in | mouse skeletal muscle tissue |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13619-1-AP targets SERCA3 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | SERCA3 fusion protein Ag4527 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ATPase, Ca++ transporting, ubiquitous |
| 别名 | ATP2A3, Calcium pump 3, SERCA3, SR Ca(2+) ATPase 3 |
| 计算分子量 | 1043 aa, 114 kDa |
| 观测分子量 | 97-110 kDa |
| GenBank蛋白编号 | BC035729 |
| 基因名称 | SERCA3 |
| Gene ID (NCBI) | 489 |
| RRID | AB_2061448 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q93084 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The SERCA, which pumps cytosolic Ca2+ into the ER, is an imperative maintainer of ER Ca2+ homeostasis. The mammalian SERCA family is comprised of three tissue-specific members, SERCA1-3. SERCA3 is present in endothelial and epithelial cells of various tissues. SERCA3 helps regulate intracellular Ca2+, which is critical for maintaining normal INS secretion and is closely linked to metabolism, neuronal plasticity, gene transcription, cell growth, differentiation, apoptosis, protein folding and carcinogenesis. Several isoforms of SERCA3 exist due to the alternative splicing.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SERCA3 antibody 13619-1-AP | Download protocol |
| IHC protocol for SERCA3 antibody 13619-1-AP | Download protocol |
| IP protocol for SERCA3 antibody 13619-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Pharmacol Astragaloside IV Attenuates Podocyte Apoptosis Mediated by Endoplasmic Reticulum Stress through Upregulating Sarco/Endoplasmic Reticulum Ca(2+)-ATPase 2 Expression in Diabetic Nephropathy. | ||
Acta Pharm Sin B Highly expressed SERCA2 triggers tumor cell autophagy and is a druggable vulnerability in triple-negative breast cancer | ||
Nat Commun Rescuing SERCA2 pump deficiency improves bone mechano-responsiveness in type 2 diabetes by shaping osteocyte calcium dynamics | ||
Front Physiol Proteomic analyses of urinary exosomes identify novel potential biomarkers for early diagnosis of sickle cell nephropathy, a sex-based study | ||
Sci China Life Sci The single-cell transcriptomic landscape of the topological differences in mammalian auditory receptors |
