ChromoTek Actin-Chromobody® plasmid (TagGFP2)

DNA plasmid encoding for anti-Actin VHH (anti-Actin Nanobody) fused to TagGFP2.

Specificity

Actin

Applications

IF, Live cell imaging

Vector type

Mammalian expression vector

Reporter

TagGFP2

Cat No : acg

Synonyms

Actin Chromobody, Chromobody



Product Information

DNA plasmid encoding for anti-Actin VHH (anti-Actin Nanobody) fused to TagGFP2.

DescriptionThe Actin-Chromobody allows imaging of actin dynamics in real-time in living cells.

• Easily transfect your cells and non-invasively visualize actin cytoskeleton in real-time

• Target and trace microfilaments in your cell line of interest

• Use Actin-Chromobody as a marker of cellular morphology and as a probe for cellular motility

• Actin-Chromobody has no notable effect cell viability or migration

ApplicationsLive cell imaging
Visualizing actin dynamics
Actin as biomarker or control
SpecificityActin microfilaments in human, rodent and plant cells, zebrafish, Toxoplasma gondii
ReporterTagGFP2
Vector typeMammalian expression vector
PromoterConstitutive CMV IE
Codon usageMammalian
SelectionKan/Neo
Nuclear localization signal (NLS)No
SequenceWith the Actin-Chromobody plasmid you obtain the sequence of the Alpaca antibody to Actin fused to TagRFP or TagGFP2, as well as the full vector sequence.
TransfectionTransfection of Chromobody plasmids into mammalian cells can be done with standard DNA-transfection methods, e.g. lipofection (Lipofectamine 2000® from Thermo Scientific), according to the manufacturer’s protocol for the transfection reagent. Please choose the transfection method that works the best for your cell type.
Microscopy techniquesWide-field epifluorescence microscopy; confocal microscopy; super-resolution microscopy e.g. STED
Storage ConditionShipped at ambient temperature. Store at -20 °C

Publications

ApplicationTitle

Nat Methods

Actin chromobody imaging reveals sub-organellar actin dynamics.

Authors - Cara R Schiavon
IF

Nat Commun

Optogenetic regulation of endogenous proteins.

Authors - Taras A Redchuk
WB

Nat Commun

Role of the nuclear membrane protein Emerin in front-rear polarity of the nucleus.

Authors - Paulina Nastały
IF

J Exp Med

IKKα controls ATG16L1 degradation to prevent ER stress during inflammation.

Authors - Michaela A Diamanti

Dev Cell

Mechanism of Long-Range Chromosome Motion Triggered by Gene Activation.

Authors - Anqi Wang
IF

EMBO J

Initiation of DNA replication requires actin dynamics and formin activity.

Authors - Nikolaos Parisis