ChromoTek Actin-Chromobody® plasmid (TagGFP2)

DNA plasmid encoding for anti-Actin VHH (anti-Actin Nanobody) fused to TagGFP2.

Vector type

Mammalian expression vector

Promoter

Constitutive CMV IE

Reporter

TagGFP2

Epitope

Actin

Cat No : acg

Synonyms

Actin Chromobody, Chromobody



Product Information

DNA plasmid encoding for anti-Actin VHH (anti-Actin Nanobody) fused to TagGFP2.

ApplicationsLive cell imaging
SpecificityActin microfilaments in human, rodent and plant cells, zebrafish, Toxoplasma gondii
Vector typeMammalian expression vector
PromoterConstitutive CMV IE
ReporterTagGFP2
Codon usageMammalian
SelectionKan/Neo
Encoded Nanobody/ VHHMonoclonal anti-Actin single domain antibody (sdAb) fragment
SequenceWith the Actin-Chromobody plasmid you obtain the sequence of the Alpaca antibody to Actin fused to TagRFP or TagGFP2, as well as the full vector sequence.
Microscopy techniquesWide-field epifluorescence microscopy; confocal microscopy; super-resolution microscopy e.g. STED
TransfectionTransfection of Chromobody plasmids into mammalian cells can be done with standard DNA-transfection methods, e.g. lipofection (Lipofectamine 2000® from Thermo Scientific), according to the manufacturer’s protocol for the transfection reagent. Please choose the transfection method that works the best for your cell type.
Storage ConditionShipped at ambient temperature. Store at -20 °C

Publications

ApplicationTitle

Front Synaptic Neurosci

Interrogating Synaptic Architecture: Approaches for Labeling Organelles and Cytoskeleton Components.

Authors - Sofiia Reshetniak
IP

Methods Mol Biol

Visualization and Quantitative Analysis of the Actin Cytoskeleton Upon B Cell Activation.

Authors - Vid Šuštar

Methods Mol Biol

Live Cell Imaging of Nuclear Actin Filaments and Heterochromatic Repair foci in Drosophila and Mouse Cells.

Authors - Colby See

Methods Cell Biol

Generation, expression and utilization of single-domain antibodies for in vivo protein localization and manipulation in sea urchin embryos.

Authors - Catherine S Schrankel

Cell Mol Bioeng

Characterization of 3D Printed Stretching Devices for Imaging Force Transmission in Live-Cells.

Authors - Carl R Mayer

J Biochem

Measuring nuclear calcium and actin assembly in living cells.

Authors - Mahira Safaralizade