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Species Reactivity
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Species Reactivity
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • WB analysis using 66129-1-Ig (same clone as 66129-1-PBS)

    WB analysis using 66129-1-Ig (same clone as 66129-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66129-1-Ig (Arginase-1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66129-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse liver using 66129-1-Ig (same clone as 66129-1-PBS)

    IP experiment of mouse liver using 66129-1-Ig (same clone as 66129-1-PBS)

    IP result of anti-Arginase-1 (IP:66129-1-Ig, 4ug; Detection:66129-1-Ig 1:10000) with mouse liver tissue lysate 2240 ug. This data was developed using the same antibody clone with 66129-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse liver using 66129-1-Ig (same clone as 66129-1-PBS)

    IF Staining of mouse liver using 66129-1-Ig (same clone as 66129-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Arginase-1 antibody (66129-1-Ig, Clone: 5D6D12 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66129-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66129-1-Ig (same clone as 66129-1-PBS)

    IF Staining of HepG2 using 66129-1-Ig (same clone as 66129-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Arginase-1 antibody (66129-1-Ig, Clone: 5D6D12 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66129-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 66129-1-Ig (same clone as 66129-1-PBS)

    IHC staining of human liver using 66129-1-Ig (same clone as 66129-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66129-1-Ig (Arginase-1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66129-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ARG1 antibody included
  • IHC staining of mouse liver using 66129-1-Ig (same clone as 66129-1-PBS)

    IHC staining of mouse liver using 66129-1-Ig (same clone as 66129-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66129-1-Ig (Arginase-1 antibody) at dilution of 1:4800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66129-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ARG1 antibody included
  • IF Staining of mouse liver using 66129-1-Ig (same clone as 66129-1-PBS)

    IF Staining of mouse liver using 66129-1-Ig (same clone as 66129-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Arginase-1 antibody (66129-1-Ig, Clone: 5D6D12 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66129-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

Arginase-1 Monoclonal antibody, PBS Only

Arginase-1 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA
Cat No. 66129-1-PBS

产品说明书

CloneNo. 5D6D12

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA

别名

ARG1, Arginase 1, liver Arginase, arginase, liver, Arg-1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy ARG1 Ready-To-Use IHC Kit. ARG1 primary antibody included.

产品信息

66129-1-PBS targets Arginase-1 in WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原 Arginase-1 fusion protein Ag8810 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 arginase, liver
别名 ARG1, Arginase 1, liver Arginase, arginase, liver, Arg-1
计算分子量236aa,25 kDa; 322aa,35 kDa
观测分子量 36 kDa
GenBank蛋白编号BC005321
基因名称 Arginase-1
Gene ID (NCBI) 383
RRIDAB_2881528
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP05089
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Arginase-1 (Liver arginase) belongs to the arginase family. ARG1 is a novel immunohistochemical marker of hepatocellular differentiation in fine needle aspiration cytology and a marker of hepatocytes and hepatocellular neoplasms. ARG1 is closely associated with alternative macrophage activation and ARG1 has been shown to protectmotor neurons from trophic factor deprivation and allow sensory neurons to overcome neurite outgrowth inhibition by myelin proteins (PMID: 20071539, PMID:12098359). It can exsit as a homotrimer and it has 3 isoforms produced by alternative splicing (PMID:16141327). Defects in ARG1 are the cause of argininemia (ARGIN). Deletion or TNF-mediated restriction of ARG1 unleashes the production of NO by NOS2, which is critical for pathogen control (PMID:27117406). Before stroke, ARG1 mainly expressed in neurons in a normal brain (PMID: 23311438). The expression of ARG1 increases in microglia/macrophages and astrocytes early after CNS injuries. ARG1 has been regarded as a marker for beneficial microglia/macrophages and possesses antiinflammatory and tissue repair properties under various pathological conditions (PMID: 26538310, PMID: 31619589).