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  • Featured Product
  • KD/KO Validated

Arginase-1 Monoclonal antibody, PBS Only

Arginase-1 Monoclonal Antibody for WB, IP, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, rat, mouse

Applications

WB, IP, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5D6D12

Cat No : 66129-1-PBS

Print datasheet

Synonyms

ARG1, Arginase 1, arginase, liver, Arginase-1, liver Arginase, Liver type arginase

验证数据展示

  • IP experiment of rat liver using 66129-1-Ig (same clone as 66129-1-PBS)

    IP experiment of rat liver using 66129-1-Ig (same clone as 66129-1-PBS)

    IP result of anti-Arginase-1 (IP:66129-1-Ig, 5ug; Detection:66129-1-Ig 1:1000) with rat liver tissue lysate 5520 ug. This data was developed using the same antibody clone with 66129-1-PBS in a different storage buffer formulation.

  • WB analysis using 66129-1-Ig (same clone as 66129-1-PBS)

    WB analysis using 66129-1-Ig (same clone as 66129-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66129-1-Ig (Arginase-1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66129-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 66129-1-Ig (same clone as 66129-1-PBS)

    IHC staining of mouse liver using 66129-1-Ig (same clone as 66129-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66129-1-Ig (Arginase-1 antibody) at dilution of 1:4800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66129-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ARG1 antibody included
  • IF Staining of HepG2 using 66129-1-Ig (same clone as 66129-1-PBS)

    IF Staining of HepG2 using 66129-1-Ig (same clone as 66129-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Arginase-1 antibody (66129-1-Ig, Clone: 5D6D12 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66129-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy ARG1 Ready-To-Use IHC Kit. ARG1 primary antibody included.

产品信息

66129-1-PBS targets Arginase-1 in WB, IP, IF, IHC, Indirect ELISA applications and shows reactivity with human, pig, rat, mouse samples.

Tested Applications WB, IP, IF, IHC, Indirect ELISA Application Description
Tested Reactivity human, pig, rat, mouse
Immunogen Arginase-1 fusion protein Ag8810 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name arginase, liver
Synonyms ARG1, Arginase 1, arginase, liver, Arginase-1, liver Arginase, Liver type arginase
Calculated Molecular Weight236aa,25 kDa; 322aa,35 kDa
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC005321
Gene Symbol ARG1
Gene ID (NCBI) 383
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP05089
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Arginase-1 (Liver arginase) belongs to the arginase family. ARG1 is a novel immunohistochemical marker of hepatocellular differentiation in fine needle aspiration cytology and a marker of hepatocytes and hepatocellular neoplasms. ARG1 is closely associated with alternative macrophage activation and ARG1 has been shown to protectmotor neurons from trophic factor deprivation and allow sensory neurons to overcome neurite outgrowth inhibition by myelin proteins (PMID: 20071539, PMID:12098359). It can exsit as a homotrimer and it has 3 isoforms produced by alternative splicing (PMID:16141327). Defects in ARG1 are the cause of argininemia (ARGIN). Deletion or TNF-mediated restriction of ARG1 unleashes the production of NO by NOS2, which is critical for pathogen control (PMID:27117406). Before stroke, ARG1 mainly expressed in neurons in a normal brain (PMID: 23311438). The expression of ARG1 increases in microglia/macrophages and astrocytes early after CNS injuries. ARG1 has been regarded as a marker for beneficial microglia/macrophages and possesses antiinflammatory and tissue repair properties under various pathological conditions (PMID: 26538310, PMID: 31619589).