AuthentiKine™ Human IFN alpha 2A ELISA Kit
Cat No : KE00207
Synonyms
IFN alpha 2, IFNA, IFNA2, IFN-alpha, IFN-alpha 2, IFN-alpha 2A, INFA2, Interferon alpha 2, Interferon alpha A, interferon, alpha 2, LeIF A
Validation Data Gallery
Product Information
KE00207 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IFN alpha 2A ELISA kit is to be used to detect and quantify protein levels of endogenous IFN alpha 2A. The assay recognizes human IFN alpha 2A. An antibody specific for IFN alpha 2A has been pre-coated onto the microwells. The IFN alpha 2A protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for IFN alpha 2A is added to detect the captured IFN alpha 2A protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | AuthentiKine™ Human IFN alpha 2A ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell culture supernatants , Human Plasma |
| Assay type | Sandwich |
| Sensitivity | 1.2 pg/mL |
| Range | 7.8-500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3440 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell culture supernatants | 96% | 85%-104% |
| Human Plasma | 98% | 82%-124% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 238.7 | 3.5 | 1.4 |
| 2 | 20 | 55.2 | 0.8 | 1.5 |
| 3 | 20 | 13.1 | 0.6 | 4.3 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 231.1 | 4.4 | 1.9 |
| 2 | 24 | 53.8 | 1.0 | 1.9 |
| 3 | 24 | 13.7 | 0.7 | 5.2 |
Background Information
Human interferon alpha-2 (IFNA2), is a member of the Type I interferon cytokine family, known for its antiviral and anti-proliferative functions. Several IFN alpha2 alleles have been described, and the best known are alpha-2a and alpha-2b. IFN alpha2 binds a plasma membrane receptor made of IFNAR1 and IFNAR2 that is ubiquitously expressed, and thus is able to act on virtually all body cells. interferon alpha 2 is effective in reducing the symptoms and duration of the common cold and in treating many types of cancer, including some hematological malignancies and solid tumors. Interferon alpha-2 products, such as interferon alpha-2a (IFNA2) are used to treat multiple diseases such as hairy cell leukemia and hepatitis c.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | IFN alpha 2, IFNA, IFNA2, IFN-alpha, IFN-alpha 2, IFN-alpha 2A, INFA2, Interferon alpha 2, Interferon alpha A, interferon, alpha 2, LeIF A |
Publications
| Species | Sample Type | Title |
|---|---|---|
Cell Rep IFNα-induced BST2+ tumor-associated macrophages facilitate immunosuppression and tumor growth in pancreatic cancer by ERK-CXCL7 signaling |