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验证数据展示

  • WB analysis using 66762-1-Ig (same clone as 66762-1-PBS)

    WB analysis using 66762-1-Ig (same clone as 66762-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66762-1-Ig (BACH1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66762-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 66762-1-Ig (same clone as 66762-1-PBS)

    WB analysis of HEK-293 using 66762-1-Ig (same clone as 66762-1-PBS)

    WB result of BACH1 antibody (66762-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BACH1 transfected HEK-293 cells. This data was developed using the same antibody clone with 66762-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 66762-1-Ig (same clone as 66762-1-PBS)

    IHC staining of human lung cancer using 66762-1-Ig (same clone as 66762-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66762-1-Ig (BACH1 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66762-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 66762-1-Ig (same clone as 66762-1-PBS)

    IHC staining of human lung cancer using 66762-1-Ig (same clone as 66762-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66762-1-Ig (BACH1 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66762-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50384-1

    Cytometric bead array standard curve of MP50384-1

    Cytometric bead array standard curve of MP50384-1, BACH1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66762-1-PBS. Detection antibody: 66762-2-PBS. Standard:Ag6011. Range: 6.25-200 ng/mL.

  • Cytometric bead array standard curve of MP50384-2

    Cytometric bead array standard curve of MP50384-2

    Cytometric bead array standard curve of MP50384-2, BACH1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66762-1-PBS. Detection antibody: 66762-3-PBS. Standard:Ag6011. Range: 6.25-200 ng/mL.

  • Cytometric bead array standard curve of MP80007-3

    Cytometric bead array standard curve of MP80007-3

    Cytometric bead array standard curve of MP80007-3, BACH1 Hybrid Matched Antibody Pair, PBS Only. Capture antibody: 66762-1-PBS. Detection antibody: 82842-2-PBS. Standard: Ag6011. Range: 0.313-40 ng/mL.

  • Featured Product
  • KD/KO验证

BACH1 Monoclonal antibody, PBS Only (Capture)

BACH1 Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA
Cat No. 66762-1-PBS

产品说明书

CloneNo. 2D12A4

宿主/亚型

Mouse / IgG1

种属反应性

human, rat

应用

WB, IHC, Cytometric bead array, Indirect ELISA

别名

2D12A4, BACH 1, BACH-1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66762-1-PBS targets BACH1 as part of a matched antibody pair:

MP50384-1: 66762-1-PBS capture and 66762-2-PBS detection (validated in Cytometric bead array)

MP50384-2: 66762-1-PBS capture and 66762-3-PBS detection (validated in Cytometric bead array)

MP80007-3: 66762-1-PBS capture and 82842-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原 BACH1 fusion protein Ag6011 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 BTB and CNC homology 1, basic leucine zipper transcription factor 1
别名 2D12A4, BACH 1, BACH-1
计算分子量 82 kDa
观测分子量 100-110 kDa
GenBank蛋白编号BC063307
基因名称 BACH1
Gene ID (NCBI) 571
ENSEMBL Gene IDENSG00000156273
RRIDAB_2882108
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO14867
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BTB and CNC homology 1, basic leucine zipper transcription factor 1(BACH1) is a heme-regulated transcriptional regulator that acts as a repressor or activator and a member of the AP-a superfamily. MafK belongs to the small Maf family that can function as transcriptional activators or repressors depending on the proteins with which they form heterodimers. BACH1 has crucial roles in both stress responses and transformation by H-RasV12. The molecular weight of expected mobility Bach1 is 100-110 kDa.