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验证数据展示

  • WB analysis using 60267-1-Ig (same clone as 60267-1-PBS)

    WB analysis using 60267-1-Ig (same clone as 60267-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60267-1-Ig (BAX antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60267-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 60267-1-Ig (same clone as 60267-1-PBS)

    IHC staining of human liver cancer using 60267-1-Ig (same clone as 60267-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60267-1-Ig (BAX antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60267-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with BAX antibody included
  • IHC staining of human liver cancer using 60267-1-Ig (same clone as 60267-1-PBS)

    IHC staining of human liver cancer using 60267-1-Ig (same clone as 60267-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60267-1-Ig (BAX antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60267-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with BAX antibody included
  • IP experiment of THP-1 using 60267-1-Ig (same clone as 60267-1-PBS)

    IP experiment of THP-1 using 60267-1-Ig (same clone as 60267-1-PBS)

    IP result of anti-BAX (IP:60267-1-Ig, 4ug; Detection:60267-1-Ig 1:6000) with THP-1 cells lysate 1520 ug. This data was developed using the same antibody clone with 60267-1-PBS in a different storage buffer formulation.

  • IHC staining of human rectal cancer using 60267-1-Ig (same clone as 60267-1-PBS)

    IHC staining of human rectal cancer using 60267-1-Ig (same clone as 60267-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 60267-1-Ig (BAX antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60267-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with BAX antibody included
  • Featured Product
  • KD/KO验证

BAX Monoclonal antibody, PBS Only

BAX Monoclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 60267-1-PBS

产品说明书

CloneNo. 4G5E8

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

Bcl-2-like protein 4, Bcl2-L-4, Apoptosis regulator BAX, 4G5E8

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy BAX Ready-To-Use IHC Kit. BAX primary antibody included.

产品信息

60267-1-PBS targets BAX in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 BAX fusion protein Ag21068 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 BCL2-associated X protein
别名 Bcl-2-like protein 4, Bcl2-L-4, Apoptosis regulator BAX, 4G5E8
计算分子量 21 kDa
观测分子量 21 kDa
GenBank蛋白编号BC014175
基因名称 BAX
Gene ID (NCBI) 581
RRIDAB_2848213
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ07812
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BAX, also named as BCL2L4, is a pro-apoptotic member of the Bcl-2 protein family, which plays a pivotal role in controlling cell life and death. Bax largely localizes to the cytoplasm of healthy cells, but accumulates on the outer mitochondrial membrane upon apoptosis induction (PMID: 9108035). BAX can commit a cell to apoptosis by translocation from the cytosol to the mitochondria and permeabilization of the outer mitochondrial membrane, which leads to the release of cytochrome c from mitochondria (PMID: 21763611). The expression of BAX is upregulated by the tumor suppressor protein p53, and BAX has been shown to be involved in p53-mediated apoptosis (PMID: 8183579).