验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IF/ICC detected in | hTERT-RPE1 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11188-2-AP targets BBS2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag1662 Product name: Recombinant human BBS2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-325 aa of BC014140 Sequence: MLLPVFTLKLRHKISPRMVAIGRYDGTHPCLAAATQTGKVFIHNPHTRNQHVSASRVFQSPLESDVSLLNINQAVSCLTAGVLNPELGYDALLVGTQTNLLAYDVYNNSDLFYREVADGANVVVLGTLGDISSPLAIIGGNCALQGFNHEGSDLFWTVTGDNVNSLALCDFDGDGKKELLVGSEDFDIRVFKEDEIVAEMTETEIVTSLCPMYGSRFGYALSNGTVGVYDKTSRYWRIKSKNHAMSIHAFDLNSDGVNELITGWSNGKVDARSDRTGEVIFKDNFSSAIAGVVEGDYRMDGHIQLICCSVDGEIRGYLPGTAEMR 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Bardet-Biedl syndrome 2 |
| 别名 | BBS, Bardet-Biedl syndrome 2 protein, Bardet Biedl syndrome 2 |
| 计算分子量 | 80 kDa |
| 观测分子量 | 80 kDa |
| GenBank蛋白编号 | BC014140 |
| 基因名称 | BBS2 |
| Gene ID (NCBI) | 583 |
| RRID | AB_2064971 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BXC9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
BBS2 is one of seven BBS proteins that form the stable core of a protein complex (BBSome complex, 438kd) which is required for ciliogenesis but is dispensable for centriolar satellite function.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for BBS2 antibody 11188-2-AP | Download protocol |
| IP protocol for BBS2 antibody 11188-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell The conserved Bardet-Biedl syndrome proteins assemble a coat that traffics membrane proteins to cilia. | ||
Cell Stem Cell Asymmetric segregation of the double-stranded RNA binding protein Staufen2 during mammalian neural stem cell divisions promotes lineage progression. | ||
J Cell Biol Ubiquitin links smoothened to intraflagellar transport to regulate Hedgehog signaling. | ||
Brain Struct Funct A novel form of ciliopathy underlies hyperphagia and obesity in Ankrd26 knockout mice. | ||
Hum Mol Genet Essential role of nephrocystin in photoreceptor intraflagellar transport in mouse. | ||
PLoS Genet The BBSome Controls Energy Homeostasis by Mediating the Transport of the Leptin Receptor to the Plasma Membrane. |