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  • Featured Product
  • KD/KO Validated

human BCL2 Polyclonal antibody

human BCL2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (7)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (3)

Conjugate

Unconjugated

Cat no : 12789-1-AP

Print datasheet

Synonyms

BCL-2, BCL2

验证数据展示

  • WB analysis of A431 using 12789-1-AP

    WB analysis of A431 using 12789-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 12789-1-AP (BCL2 antibody) at dilution of 1:9000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 12789-1-AP

    IHC staining of human tonsillitis using 12789-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12789-1-AP (BCL2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BCL2 antibody included
  • IHC staining of human tonsillitis using 12789-1-AP

    IHC staining of human tonsillitis using 12789-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12789-1-AP (BCL2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BCL2 antibody included
  • IP experiment of HL-60 using 12789-1-AP

    IP experiment of HL-60 using 12789-1-AP

    IP result of anti-human BCL2 (IP:12789-1-AP, 4ug; Detection:12789-1-AP 1:1000) with HL-60 cells lysate 4000 ug.

  • WB analysis of Jurkat cells using 12789-1-AP

    WB analysis of Jurkat cells using 12789-1-AP

    WB result of BCL2 (12789-1-AP, 1:1000) with si-Control and si-BCL2 transfected Jurkat cells.

  • FC experiment of HeLa using 12789-1-AP

    FC experiment of HeLa using 12789-1-AP

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug human BCL2 Polyclonal antibody (12789-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of Jurkat using 12789-1-AP

    IF Staining of Jurkat using 12789-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using human BCL2 antibody (12789-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

经过测试的应用

Positive WB detected inA431 cells, HepG2 cells, Jurkat cells, HEK-293 cells, HL-60 cells, U-251 cells, U-937 cells, MCF-7 cells, U2OS cells
Positive IP detected inHL-60 cells, HepG2 cells, MCF-7 cells
Positive IHC detected inhuman tonsillitis tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inJurkat cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy BCL2 Ready-To-Use IHC Kit. BCL2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:300
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12789-1-AP targets human BCL2 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, chIP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP, CoIP, chIP
Tested Reactivity human
Cited Reactivityhuman, rabbit, chicken, zebrafish, hamster, sheep, goat, fish
Immunogen human BCL2 fusion protein Ag3508 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name B-cell CLL/lymphoma 2
Synonyms BCL-2, BCL2
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 25-30 kDa
GenBank Accession NumberBC027258
Gene Symbol BCL2
Gene ID (NCBI) 596
RRIDAB_2227948
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP10415
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

1. What is Bcl-2?

Bcl-2 (B-cell lymphoma 2) is an outer mitochondrial membrane protein that via heterodimerization with BAX proteins controls apoptosis. Abnormalities of Bcl-2 activity can lead to cancer, autoimmune diseases, and schizophrenia.

2. FAQs for Bcl-2

 a. How to measure Bcl-2 and Bax apoptotic markers by western blotting

Generally, Bcl-2 protein is considered to have anti-apoptotic activity, while Bax has apoptotic activity. Comparing the ratio between Bcl-2 and Bax protein levels in different samples or cells subjected to treatments can reflect the apoptotic state of the cells. Expression of Bax can vary between cell lines and it is important to compare it to Bcl-2 levels.

b. What band size should I expect in western blotting for Bcl-2?

The molecular weight of Bcl-2 is 26 kDa.


实验方案

Product Specific Protocols
WB protocol for human BCL2 antibody 12789-1-APDownload protocol
IHC protocol for human BCL2 antibody 12789-1-APDownload protocol
IF protocol for human BCL2 antibody 12789-1-APDownload protocol
IP protocol for human BCL2 antibody 12789-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

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