验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, HepG2 cells, Jurkat cells, HEK-293 cells, HL-60 cells, U-251 cells, U-937 cells, MCF-7 cells, U2OS cells |
| Positive IP detected in | HL-60 cells, HepG2 cells, MCF-7 cells |
| Positive IHC detected in | human tonsillitis tissue, human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Jurkat cells |
| Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12789-1-AP targets human BCL2 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, chIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP, chIP |
| 经测试反应性 | human |
| 文献引用反应性 | human, rabbit, chicken, zebrafish, hamster, sheep, goat, fish |
| 免疫原 | human BCL2 fusion protein Ag3508 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | B-cell CLL/lymphoma 2 |
| 别名 | BCL-2, BCL2 |
| 计算分子量 | 26 kDa |
| 观测分子量 | 25-30 kDa |
| GenBank蛋白编号 | BC027258 |
| 基因名称 | BCL2 |
| Gene ID (NCBI) | 596 |
| RRID | AB_2227948 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P10415 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
1. What is Bcl-2?
Bcl-2 (B-cell lymphoma 2) is an outer mitochondrial membrane protein that via heterodimerization with BAX proteins controls apoptosis. Abnormalities of Bcl-2 activity can lead to cancer, autoimmune diseases, and schizophrenia.
2. FAQs for Bcl-2
a. How to measure Bcl-2 and Bax apoptotic markers by western blotting
Generally, Bcl-2 protein is considered to have anti-apoptotic activity, while Bax has apoptotic activity. Comparing the ratio between Bcl-2 and Bax protein levels in different samples or cells subjected to treatments can reflect the apoptotic state of the cells. Expression of Bax can vary between cell lines and it is important to compare it to Bcl-2 levels.
b. What band size should I expect in western blotting for Bcl-2?
The molecular weight of Bcl-2 is 26 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for human BCL2 antibody 12789-1-AP | Download protocol |
| IHC protocol for human BCL2 antibody 12789-1-AP | Download protocol |
| IF protocol for human BCL2 antibody 12789-1-AP | Download protocol |
| IP protocol for human BCL2 antibody 12789-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
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